Spectrum-effect relationships between UPLC fingerprints and bioactivities of crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi) and its three processed products on mitochondrial growth coupled with canonical correlation analysis |
| |
| Institution: | 1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;2. China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China;3. Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China;4. Department of Pharmacy, Guang Dong Pharmacy College, 68, Nanhua District, Guangzhou 51006, China;5. The Second Hospital Affiliated to Harbin Medical University, No 86, Baojian Road, Nangang District, Harbin, Heilongjiang Province, 150086, China;1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China;2. Department of Pharmacy, 302 Military Hospital of China, Beijing, 100039, China;3. China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing, 100039, China;4. Department of Integrative Medical Center, 302 Military Hospital of China, Beijing, 100039, China;1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China;2. Department of Pharmacy, 302 Military Hospital of China, Beijing, 100039, China;3. China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing, 100039, China;4. Experimental Laboratory Center, 302 Military Hospital of China, Beijing, 100039, China;1. Zhejiang Chinese Medical University, Binjiang 310053, China;2. Zhejiang Provincial Hospital of TCM, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China;1. Department of Infectious Diseases, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, 39 Shierqiao Road, Chengdu 610072, Sichuan, China;2. Department of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan, China;3. Department of Hepatology Disease, Shanghai Shuguang Hospital, Shanghai University of T.C.M., Shanghai 201203, China;1. Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China;2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China |
| |
| Abstract: | Ethnopharmacological relevance: The crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi), together with its processed products, including Yanfuzi, Heishunpian and Paofupian, are commonly applied in clinic using for thousands of years, such as collapse, syncope, rheumatic fever, painful joints and various tumors.Aim of the study: To explore the different effects of Fuzi and its processed products on energy metabolism, with mitochondria as the model with the aim of guiding the clinical use of Fuzi and its products. fingerprints of Fuzi, Yanfuzi, Heishunpian and Paofupian were established by Ultra-high Performance Liquid Chromatography (UPLC) and effects of Fuzi and its processed products on rat’s liver׳s mitochondrial metabolism were studied by microcalorimetry. Spectrum-effect relationships between UPLC fingerprints and energy metabolism of mitochondria were investigated using canonical correlation analysis (CCA).Results: Because of their inherent differences in chemical compositions, the main activities of energy metabolism of mitochondria were different among Fuzi and its processed products. The potential bioactivity sequence of the tested products was Fuzi>Heishunpian>Paofupian>Yanfuzi. Results of CCA showed that compounds mesaconitine, benzoylaconitine, and benzoylhypacoitine might be the principal active components.Conclusion: Altogether, this work provides a general model of combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Fuzi and its processed products which can offer some references for detecting principal components of traditional Chinese medicine on bioactivity to mitochondrial growth. |
| |
| Keywords: | Processed products Mitochondria UPLC fingerprints Microcalorimetry Spectrum-effect relationships |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
