两组鲍曼不动杆菌对Hep-2细胞黏附及致其凋亡情况的研究

目的研究全敏感与泛耐药两组鲍曼不动杆菌对Hep-2细胞黏附及致其凋亡情况的差异。方法选取20株全敏感和20株泛耐药鲍曼不动杆菌,调菌浓度为3×108CFU/ml,取0.5ml分别加入到Hep-2细胞株的单细胞层培养物中,放5%CO2培养箱分别培养2、8、12、16和20h,分别用于计算Hep-2细胞的细胞黏附率和细胞黏附指数,不同时间段细胞凋亡情况的分析。结果全敏感菌株对Hep-2细胞的黏附以分散广泛黏附为主,引起细胞株病变快且形态改变明显;泛耐药菌株对Hep-2细胞的黏附以局部黏附为主,易被细胞吞噬,致细胞株病变慢,诱导细胞凋亡时间较全敏感组菌株滞后。两组耐药性不同的菌株在对Hep-2细胞的平均细胞黏附率上存在显著性差异,但在平均细胞黏附指数上的差异无统计学意义。结论泛耐药鲍曼不动杆菌对Hep-2细胞表现出黏附能力和致细胞病变能力的减弱,或许是自身突变、外膜蛋白缺失及各种耐药基因插入的影响,但具体原因和机制有待进一步研究。

Adherence and Induced Apoptosis to Hep-2 Cell in Two Different Groups of Acinetobacter Baumannii Strains

Objective To study on the adherence and apoptosis to Hep-2 cells in two different groups of acinetobacter baumannii strains.Methods Totally 20 susceptible and pandrug-resistant strains were selected and 0.5ml of the bacter suspension with a concentration of 3×108CFU/ml was added into the monolayer cultures of Hep-2 cells.Then the culture was kept in the temperature of 37℃ and the atmospheric concentraion of 5% CO2 for 2,8,12,16 and 20h respectively.Finally,the adherence rate,adherence index and apoptosis situation of Hep-2 cells from different time were calculated.Results The group of susceptible strains adhereing to the Hep-2 cells was mainly in disperse and wide pattern,which caused quick cell lesion and obvious morphological change;while pandrug-resistant strains were mainly local adherence,which caused light cell lesion and slow morphological change.There was significant difference in the average rate of cell adherence,while no significant difference in the average cell adhesion index between the two groups was found.Conclusion It illustrated that the ability of adhering to Hep-2 cell and cytopathogenicity were weakened in pandrug-resistant strains.Eventhough the mechanism of adherence and pathogenicity of A.baumannii still remains to be elucidated,it was assumed that self mutation,the deficiency of outer membrane protein or the effect of inserting of various drug-resistance genes might cause the above results.