PPAR-γ在增生性瘢痕中的表达及意义

目的探讨核转录因子过氧化物酶体增殖物激活受体-γ(peroxisome proliferator-activated receptor gamma,PPAR-γ)在正常皮肤成纤维细胞和增生性瘢痕成纤维细胞中的表达及其意义。方法原代细胞培养正常皮肤成纤维细胞和增生性瘢痕成纤维细胞,应用实时定量聚合酶链式反应技术(real-time Q-PCR)检测细胞中PPAR-γm RNA的表达;Western blotting技术检测细胞中PPAR-γ蛋白的表达。结果 Real-time Q-PCR结果显示PPAR-γmRNA在正常皮肤成纤维细胞和增生性瘢痕成纤维细胞中均有表达,正常皮肤成纤维细胞中PPAR-γmRNA的表达量明显高于增生性瘢痕成纤维细胞中PPAR-γmRNA的表达量,两者之间的差异有显著性意义(P<0.05);Western blotting结果显示PPAR-γ蛋白在正常皮肤成纤维细胞和增生性瘢痕成纤维细胞中均有表达,增生性瘢痕成纤维细胞中PPAR-γ蛋白明显低于正常皮肤成纤维细胞,两者之间的差异有显著性意义(P<0.05)。结论 PPAR-γ在增生性瘢痕成纤维细胞中表达下降,提示其可能参与了增生性瘢痕的形成过程,调控PPAR-γ有望成为治疗增生性瘢痕的新靶点。

Expression and Significance of PPAR-γ in Hypertrophic Scar

Objective To investigate the expression of peroxisome proliferator-activated receptor gamma(PPAR-γ)in human normal dermal fibroblasts and hypertrophic scar fibroblasts.Methods Human normal dermal fibroblasts and hypertrophic scar fibroblasts were cultured and the expression of PPAR-γ mRNA and protein were detected by real-time Q-PCR and Western blot,respectively.Results The expression of PPAR-γ mRNA and protein were both expressed in human normal dermal fibroblasts and hypertrophic scar fibroblasts,but were lower in hypertrophic scar fibroblasts than in normal dermal fibroblasts,and the differences were significance(both P<0.05).Conclusion The expression of PPAR-γ was lower in hypertrophic scar fibroblasts than in normal dermal fibroblasts.It may involved in the formation of hypertrophic scar.Activating of PPAR-γ may be a new target for the treatment of hypertrophic scar.