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| LASIK对角膜内皮细胞酶活性影响的实验研究 | |
| 引用本文： | 孟晶,张丰菊,赵军,陈敏玲. LASIK对角膜内皮细胞酶活性影响的实验研究[J]. 中国实用眼科杂志, 2002, 20(5): 392-393 |
| 作者姓名： | 孟晶 张丰菊 赵军 陈敏玲 |
| 作者单位： | 1. 暨南大学附属第一医院眼科,510630 2. 大连医科大学附属第一医院眼科,116011 3. 肇庆市中医院眼科,526020 |
| 摘 要： | 目的 :研究准分子激光原位角膜磨镶术 (LASIK)对兔角膜内皮细胞酶活性的影响。方法 :以酶组织化学染色法测定Lasik术后 1,3 ,7,10 ,15 ,60天以及三种不同切削深度兔角膜内皮细胞乳酸脱氢酶 (LDH ) ,还原型辅酶I (NADH)的活性改变。结果 :Lasik术后不同时间组与对照组以及三种不同切削深度组的角膜内皮细胞两种酶活性比较均未见显著性差异 (P >0 0 5 )。结论 :Lasik术对角膜内皮细胞代谢无显著影响 |
| 关 键 词： | 准分子激光原位角膜磨镶术 角膜内皮细胞 LDH NADH 兔 |
| 修稿时间： | 2001-01-01 |
An enzymohistochemical study on the corneal endothelium in rabbits after excimer laser in situ keratomileusis |
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| Mengjing,Zhang Fengju ,Zhao Jun,Chen Minling. An enzymohistochemical study on the corneal endothelium in rabbits after excimer laser in situ keratomileusis[J]. Chinese Journal of Practical Ophthalmology, 2002, 20(5): 392-393 | |
| Authors: | Mengjing Zhang Fengju Zhao Jun Chen Minling |
| Affiliation: | Mengjing,Zhang Fengju *,Zhao Jun,Chen Minling ** Department of Ophthalmology,Hospital of Jinan University,Guangzhou 510630,China |
| Abstract: | To investigate the changes in latic dehydrogenase (LDH) and nicotinamide adenine dinucleotide reduced (NADH) of corneal endothelium in rabbits after excimer laser in situ keratomileusis (LASIK). Methods:36 normal rabbits were divided into six groups randomly.The three different ablation depths were performed in every experimental group with LASIK.The changes in enzymic activities after operation in the different time (1d,3d,7d,10d,15d,60d) were checked. Results:There was no significant difference in the two kinds of enzymic activities (P>0 05).For the different ablation depth after LASIK,the difference of the two kinds of enzymic activities was also not significant (P>0 05). Conclusions:There was no significant effect on the activity of LDH and NADH in the corneal endothelium of rabbits after receiving LASIK. |
| Keywords: | Excimer laser in situ keratomileusis Endothelium of cornea LDH NADH Rabbit |
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