| 白介素-23对破骨细胞分化及功能直接调节作用的实验研究 |
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| 引用本文： | 陈莉,WEI Xiao-Qing,Bronwen Evans,Daniel Aeschlimann. 白介素-23对破骨细胞分化及功能直接调节作用的实验研究[J]. 北京口腔医学, 2010, 18(6): 307-310. DOI: 10.3969/j.issn.1006-673X.2010.06.003 |
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| 作者姓名： | 陈莉 WEI Xiao-Qing Bronwen Evans Daniel Aeschlimann |
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| 作者单位： | 首都医科大学口腔医学院正畸科,100050;Matrix Biology and Tissue Repair Research Unit,School of Dentistry,Cardiff University,UK |
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| 基金项目： | 国家留学基金委博士后奖学金 |
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| 摘 要： | 目的检测白介素-23（IL-23）是否对破骨细胞分化及功能有直接调节作用。方法在体外培养小鼠破骨细胞的过程中加入不同浓度的IL-23,观察破骨细胞形成数量及吸收陷窝的变化以及组织蛋白酶-K mRNA的表达变化。同时,以RAW264.7诱导破骨细胞,采用RT-PCR及FACS方法检测IL-23刺激下,RANK mRNA及蛋白的表达变化情况。结果 IL-23作用下,小鼠破骨细胞数量增加,形成的吸收陷窝增多,组织蛋白酶-K mRNA的表达增强。同时在IL-23刺激下,破骨前体细胞表面RANK mRNA及蛋白的表达增强。结论 IL-23可以通过调高破骨前体细胞表面RANK的表达,直接促进小鼠破骨细胞分化。
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| 关 键 词： | 骨吸收 IL-23 RANK 破骨细胞 组织蛋白酶K |
The regulation effect of IL-23 on osteoclast differentiation and function |
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| CHEN Li,WEI Xiao-Qing,Bronwen Evans,Daniel Aeschlimann. The regulation effect of IL-23 on osteoclast differentiation and function[J]. Beijing Journal Of Stomatology, 2010, 18(6): 307-310. DOI: 10.3969/j.issn.1006-673X.2010.06.003 |
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| Authors: | CHEN Li WEI Xiao-Qing Bronwen Evans Daniel Aeschlimann |
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| Affiliation: | CHEN Li,WEI Xiao-qing,Bronwen Evans,Daniel Aeschlimann.Department of Orthodontics,Capital Medical University School of Stomatology,Beijing 100050,China |
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| Abstract: | Objective To examine the effect of IL-23 on regulating osteoclast differentiation and function.Methods Osteoclasts from mouse bone marrow and RAW264.7 cell line were cultured in vitro.The number of osteoclast in presence of IL-23 at different concentration was counted.Dentin slices were used to observe pits formation.RT-PCR and FACS were used to examine RANK and cathepsin K expression.Results The number of osteoclasts and pits formation increased when stimulated by IL-23.RANK and cathepsin K expression were... |
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| Keywords: | Bone resorption IL-23 RANK Osteoclast Cathepsin K |
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