透射电子显微镜病理诊断样品制备标准化技术

透射电子显微镜病理诊断技术已广泛用于临床病理诊断中。作者根据国内的实际情况，以充分满足临床病理诊断的要求为中心，针对不同的标本建立了一套实用的电镜病理诊断样品制备标准化技术。该技术包括各种试剂的制备与贮存，取样的要求，５小时包埋程序，２４小时包埋程序，液体标本（穿刺细胞活检标本）包埋程序以及利用石蜡包埋块、石蜡切片制作电镜标本程序等。电镜观察结果显示，经上述方法制备的电镜样品，其组织和细胞的超微结构清晰，完全可以满足临床病理诊断需要。文内还对该技术中具有实用价值的关键步骤进行了讨论

The Standardized Techniques for Preparing Smaples Used in Pathologic Diagnostic Transmission Electron Microscopy

Transmission electron microscopy (TEM) has been used widespreadly in the field of diagnostic pathology. Based on the practical domestic condition and aimed at fully satisfying the needs of the pathologic diagnostic TEM, a set of standardized techniques were established for preparing different kinds of samples. The techniques included the preparation and storage of fixatives and embedding media, the requirment of sampling, the 5h and 24h hembedding procedures for fresh specimens, the embedding procedure for suspension specimens and specimens collected from fine needle aspiration biopsy, as well as the procedures for preparing samples taken from paraffin embedded blocks or paraffin sections. In practical use, we found that the advantage of these techniques was that the ultrastructures of the tissues and cells were well preserved and they perfectly met the needs for diagnosis of tumors, kidney diseases, et al. The main points of the standardized techniques have been discussed in detail in this paper.