Cajal间质细胞和P物质在糖尿病大鼠结肠组织中的改变

目的：分析糖尿病大鼠结肠组织中Cajal间质细胞和P物质的分布及表达变化,探讨Cajal间质细胞和P物质在糖尿病结肠功能紊乱发病中的作用.方法：建立糖尿病大鼠模型,免疫组化检测Cajal间质细胞和P物质在糖尿病大鼠结肠组织内的分布和表达.结果：Cajal间质细胞和P物质主要分布在大鼠近端和远端结肠的肌间神经丛,糖尿病大鼠结肠组织内Cajal间质细胞和P物质的表达较正常大鼠均明显减少（P＜0.05）.结论：糖尿病大鼠结肠组织内Cajal间质细胞和P物质表达减少,推测与糖尿病大鼠结肠功能紊乱有一定相关性.

The quantity study of interstitial cells of Cajal and substance P in colon tissue of diabetic rats with gastrointestinal dysfunction using immunohistochemical stain

Objective: To investigate the changes of interstitial cells of Cajal and the expression of substance P in colon tissue of diabetic rats with gastrointestinal dysfunction and to discuss the possible mechanism of gastrointestinal dysfunction associated with diabetes mellitus.Methods: Thirty SD rats were randomly divided into normal control group(n = 10) and diabetic model group(n = 20).Rats in diabetic model group were made into diabetic models with intraperitoneal injection of streptozotocin(STZ)(80 mg/kg) for only one time.and those in normal control group were intraperitoneally injected with the equal Citric Acid buffer.The distribution of interstitial cells of Cajal and the expression of substance P in the proximal and distal colon tissue were detected by immunohistochemistry.Results: The immunoreaction positive products of interstitial cells of Cajal and substance P were mainly distributed in the myenteric plexus of the colon tissue,and the expression of interstitial cells of Cajal and substance P in the proximal and distal colon tissue of diabetic rats were decreased(P < 0.05).Conclusion: Decreased expression of interstitial cells of Cajal and substance P in the colon tissue might play an important role in the gastrointestinal dysfunction associated with diabetic mellitus.