基于网络药理学对参麦注射液缓解CIPN作用机制分析

目的 探讨参麦注射液缓解化疗所致周围神经毒性(CIPN)的作用机制。方法 借助ETCM及TCMSP检索红参及麦冬的化学成分和作用靶点，通过OMIM、Disgenet、GeneCards等多个数据库查询与CIPN相关的基因。运用Cytoscape软件构建化合物-靶点网络，并利用String工具获得PPI网络筛选出核心靶点，最后通过Metascape对PPICN的靶蛋白进行基因富集分析。结果 参麦-靶点网络包含38个化合物和相应靶点105个；PPI核心网络包含23个蛋白；GO分析得到条目34个，KEGG通路富集筛选得到9条信号通路（P＜0.01）。结论 参麦注射液中对CIPN关键活性成分源自麦冬的组分占比60%；GO富集分析与KEGG通路分析结果显示参麦注射液通过多靶点与多通路的协同作用缓解CIPN。

Mechanism of Shenmai Injection in alleviating CIPN based on network pharmacology

Objective To investigate the mechanism of Shenmai Injection in alleviating chemotherapy-induced peripheral neurotoxicity(CIPN).Methods The chemical constituents and action targets of Radix Ginseng Rubra and Ophiopogon were searched by ETCM and TCMSP, and the CIPN-related genes were searched by OMIM, Disgenet and GeneCards databases.Cytoscape 3.9.1 was used to construct the compound-target (gene) network, and String tool was used to obtain protein interaction (PPI) network to screen out the core targets.Finally, gene enrichment analysis of PPICN target protein was carried out by Metascape.Results The compound target network consisted of 38compounds and 105 corresponding targets. PPI core network contains 23 proteins.34 items were obtained by GO analysis, and 9 signaling pathways were obtained by KEGG pathway enrichment analysis (P<0.01). Conclusion 60% of CIPN components in Shenmai injection were from Ophiopogon japonicus.GO enrichment analysis and KEGG pathway analysis showed that Shenmai injection alleviated CIPN through synergistic action of multi-target and multi-pathway.