纳米银凝胶与纳米银水凝胶在模拟体液中的释放特征

背景：国内外研究显示,纳米银颗粒可在体内迁移、蓄积,并最终导致细胞毒性和基因毒性,且毒性可由于纳米银的蓄积而逐渐扩大。目的：观察纳米银凝胶和纳米银水凝胶两类产品在模拟体液中的释放特征。方法：取来自6个厂家的6种纳米银产品(3种纳米银凝胶和3种纳米银水凝胶),将其浸泡在模拟体液中,提取不同时间点的纳米银凝胶和纳米银水凝胶释放液,利用超高压微波消解系统消解并定容,再用原子吸收光谱仪检测各消解液中的银含量,根据银含量检测结果分析总结两类产品在模拟体液中的释放特征。结果与结论：3种纳米银凝胶在模拟体液中的释放速率分别为3.59,4.30,1.17 g/min,达到动态平衡的时间分别为40,35,70 min;3种纳米银水凝胶在模拟体液中的释放速率分别为5.14,5.31,4.86 g/s,达到动态平衡的时间均为100 s。提示由于载体基质的不同,纳米银水凝胶的释放速率要明显高于纳米银凝胶;不同纳米银厂家由于工艺的不同,纳米银凝胶的释放速率亦有所不同,而纳米银水凝胶的释放速率区别不大。

Release characteristics of nano-silver gel versus nano-silver hydrogel in simulated body fluid

BACKGROUND:Researches at home and abroad demonstrate that silver nanoparticles can migrate and accumulate in vivo, eventually leading to cytotoxicity and genotoxicity which increases with the accumulation level.    OBJECTIVE:To observe the release characteristics of nano-silver gel and nano-silver hydrogel in simulated body fluid.METHODS:Three kinds of nano-silver gel and three kinds of nano-silver hydrogel from six manufacturers were soaked in simulated body fluid, the release liquid was extracted at different time points, and then harvested for silver content analysis by using the extra high pressure microwave and the atom absorption spectrum instrument, at last, the release characteristics of these were analyzed and summarized.RESULTS AND CONCLUSION:In the simulated body fluid, the release rate of three kinds of nano-silver gel was 3.59, 4.30 and 1.17 g/min respectively, the time to reach dynamic equilibrium was 40, 35, 70 minutes respectively; by comparison, the release rate of three kinds of nano-silver hydrogel was 5.14, 5.31 and 4.86 g/s respectively, the time to reach dynamic equilibrium was all 100 seconds. Due to the difference of carrier matrix, the release rate of nano-silver hydrogel was significantly higher than the nano-silver gel; different manufacturers and different techniques of silver nanoparticles, may lead to altered release rates of nano-silver gel, while the release rate of nano-silver hydrogel was not different.