软骨损伤后基质细胞衍生因子1的表达

背景：研究表明基质细胞衍生因子1对骨髓间充质干细胞的迁移、聚集有影响。目的：观察软骨损伤后不同时间损伤修复区组织基质细胞衍生因子1的表达以及与骨髓间充质干细胞迁移的关系。方法：建立兔软骨损伤模型,分别于建模后2,5,7,10,14,28 d取损伤灶及边缘区组织,检测基质细胞衍生因子1表达和体外细胞迁移实验,观察基质细胞衍生因子1对骨髓间充质干细胞、软骨细胞的迁移影响。结果与结论：基质细胞衍生因子1的表达呈现出时间变化的趋势,软骨损伤后第7天达到高峰(P < 0.05)。体内移植的骨髓间充质干细胞主要聚集在软骨损伤灶周围,但在封闭CXC趋化因子受体4后,此聚集现象逐渐减弱(P < 0.05)。结果证实,局部组织中基质细胞衍生因子1的表达在软骨损伤早期明显升高,对骨髓间充质干细胞向软骨损伤修复区迁移有重要作用。

Expression of stromal cell-derived factor-1 after articular cartilage injury

BACKGROUND:Studies have demonstrated that stromal cell-derived factor-1 (SDF-1) can influence the migration and aggregation of bone marrow mesenchymal stem cells (BMSCs).OBJECTIVE:To observe the expression of SDF-1 at lesion regions at different time after cartilage injury and to investigate the correlation between SDF-1 expression and BMSCs migration. METHODS:Rabbits models of articular cartilage injury were established. Focal osteochondral lesions were collected 2, 5, 7, 10, 14 and 28 days after injury. The influence of SDF-1 expression on the migration of BMSCs and chondrocytes was observed. RESULTS AND CONCLUSION:SDF-1 expression was significantly increased and peaked at the seventh day (P < 0.05). BMSCs migrated toward articular cartilage injury tissue, while they were significantly inhibited by the inhibitor of CXCL4. SDF-1 expression increases in the early phase after articular cartilage injury, and plays an important role in chemoattracting MSCs to injured lesions.