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| LMP1-CTAR3对鼻咽癌干细胞SP18生长的影响 | |
| 作者姓名： | 全 胜 庄英帜 张志伟 刘重元 姜 浩 赵 强 朱建思 董 琳 |
| 作者单位： | 1南华大学附属第一医院肿瘤内科，湖南省衡阳市 421001 2湖南环境生物职业技术学院，湖南省衡阳市 421001 3南华大学医学院肿瘤研究所，湖南省衡阳市421001 4南华大学附属第一医院病理科，湖南省衡阳市421001 |
| 基金项目： | 湖南省高校创新平台开放基金(10K052)，湖南省重点学科建设基金(2006-180)，湖南省研究生科研创新项目(CX2010B380)，湖南省大学生研究性学习和创新性实验计划项目(CXSY-SJ-09007)。 |
| 摘 要： | 背景：课题组前期构建了CTAR3缺失突变型LMP1(LMP1△232-351)表达载体，初步了解该活性区在LMP1发挥作用中扮演十分重要的角色。
目的：观察LMP1-CTAR3对鼻咽癌干细胞SP18生长的影响。
方法：采用逆病毒感染方式，建立稳定表达LMP1及CTAR3缺失突变型LMP1(LMP1△232-351)的鼻咽癌干细胞SP18细胞系，通过细胞生长曲线、软琼脂集落形成实验和平皿克隆形成实验观察LMP1△232-351对鼻咽癌干细胞SP18生长的影响，然后选用流式细胞术检测细胞周期，计算细胞增殖指数。另外，利用Western-blot检测细胞中JAK3蛋白的磷酸化水平。
结果与结论：①与SP18-LMP1细胞比较，SP18-LMP1△232-351细胞生长速度减慢，增殖指数降低(P < 0.01)。②在SP18- LMP1△232-351细胞中JAK3的磷酸化水平低于SP18- LMP1细胞。结果说明LMP1-CTAR3可能通过影响SP18细胞中JAK3的磷酸化，下调细胞的增殖指数，降低促细胞增殖的能力。 |
| 关 键 词： | 鼻咽癌干细胞SP18 LMP1 CTAR3 生长增殖 JAK3蛋白 |
| 收稿时间： | 2011-07-26 |
The growth of nasopharyngeal cancer stem cell SP18 affected by carboxy terminal activating region-3 of the Epstein-Barr virus encoded latent membrane protein 1 |
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| Authors: | Quan Sheng Zhuang Ying-zhi Zhang Zhi-wei Liu Zhong-yuan Jiang Hao Zhao Qiang Zhu Jian-si Dong Lin |
| Institution: | 1Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China 2Hunan Environment- Biological Polytechnic College, Hengyang 421001, Hunan Province, China 3Cancer Research Institute of Medical College, University of South China, Hengyang 421001, Hunan Province, China 4Department of Pathology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China |
| Abstract: | BACKGROUND: Carboxy terminal activating region-3 of the Epstein-Barr virus encoded latent membrane protein 1 (LMP1-CTAR3) has been constructed in the previous studies. CTAR3 plays an important role in LMP1. OBJECTIVE:To observe the effect of LMP1-CTAR3 on the growth of nasopharyngeal cancer stem cell SP18. METHODS:We adopted the method of retroviruses infection and established the nasopharyngeal cancer stem cell SP18 of stable expression LMP1 and LMP1△232-351. Then, we made use of cell growth curve, plate clone formation and soft agar colony experiment to observe the growth of nasopharyngeal cancer stem cell SP18 affected by LMP1△232-351. Meanwhile, we detected the cycle of SP18-LMP1△232-351 cell by flow cytometry and calculated cellular proliferation index. In addition, Western-blot detection was used to determine phosphorylation JAK3 protein. RESULTS AND CONCLUSION:Compared with SP18-LMP1 cells, the growth velocity and proliferation index of SP18-LMP1△232-351 cells obviously decreased (P < 0.01). The expression of phosphorylation JAK3 protein in SP18-LMP1△232-351 cells was lower than that in SP18-LMP1 cells. LMP1-CTAR3 probably affected the expression of phosphorylation JAK3 protein in SP18 to decrease cellular proliferation index and reduce the ability of cell proliferation promoted by LMP1. |
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