尿趋化因子IP-10和Fractalkine在移植肾急性排斥反应早期诊断中的应用

背景：相关研究已证实,趋化因子IP-10、Fractalkine在器官移植后急性排斥反应过程中发挥着重要的作用。目的：检测尿液中IP-10和Fractalkine水平变化,并结合肾组织活检病理,探讨尿液中IP-10 和Fractalkine在移植肾急性排斥反应早期诊断中的意义。方法：106例同种异体肾移植患者,根据移植后临床表现、实验室检查及肾穿刺组织病理学检查结果,分为急性排斥反应组(n=16)和非急性排斥反应组(n=90);另选择健康志愿者作为正常对照组。用双抗体夹心酶联免疫吸附试验检测尿IP-10和Fractalkine浓度变化。结果与结论：急性和非急性排斥反应组患者在移植后的尿IP-10及Fractalkine的表达水平均较移植前明显升高,但非急性排斥反应组在移植后7 d呈下降趋势,至第11天降至移植前水平,而急性排斥反应组则持续高表达,IP-10在移植后第1天和Fractalkine在移植后第3天即与急性排斥反应组比较,差异有显著性意义(P < 0.05)。提示,肾移植后尿液中IP-10和Fractalkine水平的检测对于急性排斥反应发生的早期诊断和早期治疗具有重要意义。

Application of urine IP-10 and Fractalkine in early diagnosis of acute rejection following renal transplantation

BACKGROUND:Studies have confirmed that urine IP-10 and Fractalkine play an important role in acute rejection following organ transplantation.OBJECTIVE:To investigate early diagnosis and post-treatment expression of urine IP-10 and Fractalkine in the acute rejection after renal transplantation, through detecting the association of the urine IP-10 and Fractalkine variation according to some cases of nephridial tissue biopsy. METHODS:According to the postoperative clinical manifestation, laboratory examination and renal biopsy histopathological examination results, we divided 106 patients with renal transplantation allograft patients into acute rejection group (n  = 16) and the stable group (n  = 90). Healthy volunteers were chosen as normal control group (n = 10). IP-10 and Fractalkine concentration changes were measured by double antibody sandwich enzyme-linked immunosorbent adsorption. RESULTS AND CONCLUSION:The urine level of IP-10 and Fractalkine in patients with renal transplantation post-operation was significantly higher than pre-operation group. But one week post-operative, the urine level of IP-10 and Fractalkine was declined in the stable group and to preoperative level after 11 days, and acute rejection group continued high expression. IP-10 with first day postoperative and Fractalkine with third day postoperative compared with stable group showed significant difference  (P < 0.05). It is confirmed that to detect the urine levels of IP-10 and Fractalkine in patients with renal transplantation pre-operation and post-operation has certain clinical significance to early diagnose and treat acute rejection.