仙珍骨宝胶囊对去卵巢大鼠股骨颈骨代谢的影响

背景：用骨组织形态计量学方法探讨中药对去卵巢大鼠股骨颈骨质疏松的影响，可为采用中药防治绝经后妇女骨质疏松性股骨颈骨折提供实验依据。目的：观察仙珍骨宝胶囊对去卵巢大鼠股骨颈松质骨的影响。方法：3月龄SD雌鼠随机分为4组：基础对照组于实验开始时处死取材，去卵巢组和仙珍骨宝组去卵巢造模，仙珍骨宝组在去卵巢后灌胃仙珍骨宝，去卵巢组和年龄对照组灌胃生理盐水，90 d后处死，取股骨颈经不脱钙骨制片进行骨组织形态计量学参数测量。结果与结论：与年龄对照组比较，去卵巢大鼠静态参数的骨小梁面积百分数和骨小梁数量明显减少(P < 0.01)，骨小梁间隙明显增大(P < 0.01)；动态参数的每毫米破骨细胞数和破骨细胞贴壁表面长度明显增加(P < 0.01)，骨矿化沉积率明显减少(P < 0.01)。说明去卵巢能导致大鼠股骨颈骨量显著减少。给予仙珍骨宝治疗后，大鼠的骨小梁厚度及骨小梁面积明显增加(P < 0.05)，每毫米破骨细胞数和破骨细胞贴壁表面长度有所减少，标记周长百分数则有所增加。说明仙珍骨宝能阻止去卵巢所致的大鼠股骨颈骨量丢失。

Effects of Xianzhen Gubao capsules on bone metabolism of the femoral neck in ovariectomized rats

BACKGROUND:Studies concerning effects of traditional Chinese medicine on osteoporosis of the femoral neck in ovariectomized rats using bone histomorphometry methods can provide basis for the traditional Chinese medicine treatment on femoral neck fracture in postmenopausal women with osteoporosis. OBJECTIVE:To explore the effects of capsule of Xianzhen Gubao on cancellous bone of the femoral neck in ovariectomized rats. METHODS:Female Sprague Dawley rats with 3-month old were randomly divided into the basal control, aging control, ovariectomized and Xianzhen Gubao groups. In the later two groups, rats were prepared for ovariectomized models and intragastric administration of saline and Xianzhen Gubao respectively. Rats in the aging control group were received intragastric administration of saline. After 90 days, all rats were killed and their femoral neck were processed to undecalcified sections and measured by automatic image-analysis system for bone histomorphometry.RESULTS AND CONCLUSION:Compared with aging control group, the percent trabecular area and trabecular number in the ovariectomized group were obviously decreased (P < 0.01), the trabecular separation increased (P < 0.01), while the osteoclast number per mm and percent osteoclast surface perimeter were notably increased (P< 0.01), and the mineral apposition rate were reduced (P < 0.01). The results demonstrated that ovariectomy would markedly reduce the trabecular bone of femoral neck. The capsule of Xianzhen Gubao can prevent the trabecular bone loss of the neck of femur in ovariectomized rats.