金乌骨通胶囊对成骨细胞分泌白细胞介素1，6的影响

背景：课题组以往研究发现,金乌骨通胶囊对绝经后骨质疏松症具有明显的治疗作用,但其具体的机制尚不十分清楚。目的：观察金乌骨通胶囊含药血清对成骨细胞分泌白细胞介素1,6的影响。方法：切除雌性SD大鼠双侧卵巢制备去卵巢血清,灌胃给予去卵巢大鼠金乌骨通胶囊制备去卵巢含药血清。取第3代生长状况良好的出生24 h内SD大鼠的成骨细胞,分别加入正常雌性SD大鼠血清、去卵巢血清和去卵巢含药血清培养72 h。结果与结论：酶联免疫吸附法检测显示,与正常血清组比较,去卵巢血清组成骨细胞白细胞介素1、白细胞介素6的表达明显升高(P < 0.01);与去卵巢血清组比较,去卵巢含药血清组白细胞介素1和白细胞介素6的表达明显降低(P < 0.05),与正常血清组比较,则无明显差异。提示金乌骨通胶囊可能通过抑制成骨细胞骨白细胞介素1和白细胞介素6的表达来实现治防治骨质疏松症的目的。

Effects of Jinwugutong capsule on the expression of interleukin1, 6 in osteoblasts

BACKGROUND:Our previous studies have shown that Jinwugutong capsule exhibits obvious effects on postmenopausal osteoporosis. OBJECTIVE:To investigate the effects of Jinwugutong capsule-containing serum on the expression of interleukin (IL)-1 and IL-6 in osteoblasts. METHODS:Bilateral ovaries of female Sprague-Dawley (SD) rats were removed to prepare ovarian serum. Ovariotomized (OVX) rats were intragastrically administered Jinwugutong capsule to prepare ovarian medicated serum. Passage 3 osteoblasts which were harvested from rats born within 24 hours were cultured with normal female rat serum (normal serum group), OVX rat serum (OVX serum group) and OVX rat Jinwugutong capsule-containing serum (OVX drug-containing serum group) for 72 hours separately.   RESULTS AND CONCLUSION:ELISA results showed that IL-1 and IL-6 expression in the osteoblasts was significantly increased in the OVX serum group than in the normal serum group (P < 0.01); IL-1 and IL-6 expression in the osteoblasts was significantly decreased in the OVX drug-containing serum group than in the OVX serum group, but there was no significant difference in IL-1 and IL-6 expression between normal serum group and OVX drug-containing serum group. These findings suggest that Jinwugutong capsule can prevent and treat osteoporosis by inhibiting IL-1 and IL-6 expression in osteoblasts.