构建大脑中动脉阻塞脑缺血模型大鼠的类型分析

背景：线栓法造成短暂性大脑中动脉阻塞是研究大鼠局灶性脑缺血普遍使用的模型制作方法。但制作大鼠脑缺血模型的类型存在一定差异,可能导致实验结果的偏差。目的：分析大脑中动脉阻塞线栓法制作大鼠脑缺血模型的类型及其影响因素。方法：雄性SD大鼠166只,参照Longa线栓法造模,术后24 h行MRI扫描,根据扫描结果将大鼠分成皮质梗死组、皮质下梗死组及无梗死组,分析造模时线栓插入的深度。结果与结论：皮质梗死组、皮质下梗死组和无梗死组大鼠的线栓插入深度分别为(19.9±0.9),(19.0±1.1)和(17.7±1.3) mm,皮质梗死组大鼠的线栓插入最深,而无梗死组的线栓插入最浅(P < 0.01)。提示插入深度不同导致的大鼠脑梗死的类型也不同,线栓插入越深,皮质梗死的概率可能越大。

Different types of rat cerebral ischemia models after middle cerebral artery occlusion

BACKGROUND:Suture method caused transient middle cerebral artery occlusion (MCAO) is the commonly used method to study focal cerebral ischemia. However, differences exist in cerebral ischemia models may lead to deviation of the experimental results. OBJECTIVE:To analyze the types of cerebral ischemia in rats after MCAO method and its influential factors in different types. METHODS:Referring to Longa’s method, 166 rats were prepared for MCAO models and scanned by MRI at 24 hours after the surgery. The rats were divided to cortex infarction group, subcortical infarction group and non-infarction group according to the scan results, and to analyze the insertion depth of suture in the surgery. RESULTS AND CONCLUSION:The insertion depth were (19.9±0.9), (19.0±1.1) and (17.7±1.3) mm in the cortical infarction group, subcortical infarction group and non-infarction group, respectively. There was significant difference in the depth of suture insertion between the three groups of rats (P < 0.01). It is indicated that different types of cerebral infarction in rats may be due to the different insertion depths of suture. The insertion depth of suture is deeper into the blood vessel, and the risk of cortical infarction may be higher.