骨形态发生蛋白2复合脂肪源性干细胞修复兔缺血性股骨头坏死

背景：骨形态发生蛋白2和脂肪源性干细胞都有很强的成骨能力,对于缺血性股骨头坏死的修复有重要意义。 目的：观察骨形态发生蛋白2体内复合脂肪源性干细胞成骨治疗兔股骨头缺血坏死的疗效。 方法：48只新西兰大白兔采用液氮冷冻法造模,随机分为对照组,髓芯减压组,脂肪源性干细胞组,骨形态发生蛋白2复合脂肪源性干细胞组。 结果与结论：脂肪源性干细胞组与骨形态发生蛋白2复合脂肪源性干细胞组兔股骨头坏死区域的组织修复旺盛,新生骨小梁较多且逐步强化,X射线未见明显的囊性变和股骨头塌陷发生。在8周时骨形态发生蛋白2复合脂肪源性干细胞组骨小梁及骨髓组织生长较单纯脂肪源性干细胞组更旺盛。表明骨形态发生蛋白2因子复合脂肪源性干细胞植入股骨头坏死模型后成骨较多,修复坏死区域功能较强。

Bone morphogenetic protein-2 combined with adipose derived stem cells for treatment of avascular necrosis of the femoral head in rabbits

BACKGROUND:Adipose derived stem cells (ADSCs) and bone morphogenetic protein-2 (BMP-2) have a strong osteogenic capability that is important for the repair of avascular necrosis of the femoral head. OBJECTIVE:To observe the combined effects of ADSCs and BMP-2 on avascular necrosis of the femoral head. METHODS:The avascular femoral head necrosis models were established in 48 rabbits using liquid nitrogen. The rabbits were divided into control group, core decompression group, ADSCs group and BMP-2+ADSCs group randomly.  RESULTS AND CONCLUSION: Tissue repair of femoral head necrosis in ADSCs group and BMP-2+ADSCs group was vigorous. There were more newly formed trabeculae, which were strengthened. X-ray showed no obvious cystic degeneration and collapse of the femoral head. The growth of trabeculae and bone marrow in BMP-2+ADSCs group was more vigorous than that in ADSCs group at 8 weeks. It is indicated that BMP-2 combined with ADSCs have a stronger repairable ability in necrosis of the femoral head necrosisl.