脑缺血再灌注海马齿状回神经干细胞Mash-1的表达

背景：Mash-1在中枢神经系统和周围神经系统发育中都有表达，对神经元和神经胶质细胞的分化都有很重要的作用。目的：检测Mash-1在大鼠脑缺血再灌注海马组织神经干细胞中的表达及其变化。方法：建立大脑中动脉栓塞制作大鼠脑缺血再灌注模型。实验分为假手术组、缺血再灌注3，7，14，21，28 d组。结果与结论：免疫组织化学检测结果，随着脑缺血再灌注第3天海马神经元BrdU阳性细胞明显增多，第7天达高峰，然后逐渐减少。Mash-1反应产物随脑缺血再灌注时间延长逐渐增多，第21天表达最强，以后表达逐渐减少。提示Mash-1呈时间依赖性表达，与神经干细胞增殖分化进程相吻合，说明其在神经干细胞晚期分化中起重要调控作用。

Expression of Mash-1 in hippocampal neural stem cells after cerebral ischemia/reperfusion injury in rats

BACKGROUND: Mash-1 is expressed during the development of the central nervous system and peripheral nervous system, and plays an important role in neurons and glial cell differentiation.OBJECTIVE:To investigate the expression of Mash-1 in the hippocampus after cerebral ischemia/reperfusion in rats.METHODS: A novel model of cerebral ischemia/reperfusion was established with the method of middle cerebral artery occlusionin rats. The rats were divided into sham-operation group, and ischemia/reperfusion groups of 3, 7, 14, 21 and 28 days.RESULTS AND CONCLUSION:After 3 days, BrdU positive cells in the hippocampus were obviously increased. To the 7th day, BrdU positive cells were more than those at any time. Mash-1 reaction products were increased gradually with the time of cerebral ischemia/reperfusion, reached the peak on day 21, and then gradually decreased. The expression of Mash-1 is in a time-depended manner and corresponds to the proliferation and differentiation of neural stem cells, suggesting that Mash-1 can play the important effect in regulating the neural stem cell differentiation in the late stage.