干细胞生长因子和粒细胞集落刺激因子与心肌梗死大鼠体内骨髓间充质干细胞的迁移

背景：外周静脉移植间充质干细胞只有1%~5%的移植细胞能归巢到心肌梗死区域。目的：观察干细胞生长因子、粒细胞集落刺激因子对骨髓间充质干细胞归巢的影响。方法：采用贴壁培养法分离培养SD大鼠骨髓间充质干细胞,取传至3~5代细胞。建立SD大鼠急性心肌梗死模型,干细胞生长因子组、粒细胞集落刺激因子组、干细胞生长因子+粒细胞集落刺激因子组在骨髓间充质干细胞移植前3 d和移植后3 d单独或混合皮下注射干细胞生长因子、粒细胞集落刺激因子,骨髓间充质干细胞组不注射细胞因子。结果与结论：荧光显微镜下观察,骨髓间充质干细胞迁移至心肌梗死组织,骨髓间充质干细胞组、干细胞生长因子组、粒细胞集落刺激因子组迁移至心肌梗死区的骨髓间充质干细胞数量没有明显的区别(P > 0.05),干细胞生长因子+粒细胞集落刺激因子组的骨髓间充质干细胞数量明显高于其他3组(P < 0.05)。免疫荧光组织化学显示,植入的部分骨髓间充质干细胞表达心肌特异蛋白cTnI。结果说明干细胞生长因子和粒细胞集落刺激因子两种细胞因子联合应用可以促进骨髓间充质干细胞归巢至心肌梗死区域,在体内微环境的诱导下,骨髓间充质干细胞能够转化为心肌样细胞。

Stem cell factor and granulocyte colony-stimulating factor affect the migration of bone marrow mesenchymal stem cells in rats with myocardial infarction

BACKGROUND:Only 1%-5% of the transplanted mesenchymal stem cells (MSCs) home to the myocardial infarction area through peripheral venous transplantation.OBJECTIVE:To investigate the effects of stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) on MSCs homing.METHODS:MSCs were isolated from SD rats, and purified by adhesive-screening method. MSCs at passages 3-5 were assigned to 4 groups: groups of MSCs, SCF, G-CSF and SCF+G-CSF.RESULTS AND CONCLUSION:Under fluorescence microscope, the number of MSCs migrating to the myocardial infarction region had significant difference among MSCs group, SCF group and G-CSF group (P > 0.05), but SCF+G-CSF group had the higher number of MSCs migrating to the myocardial infarction region than other three groups (P < 0.05). Immunofluorescence histochemistry showed that some MSCs express cardiac-specific protein cTnI. Combination of SCF and G-CSF can promote MSCs homing, and induced by microenvironment in vivo, MSCs can convert into cardiomyocyte-like cells.