| 大蒜素对HL-60细胞端粒酶活性的影响 |
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| 引用本文： | 王旭光,陈根殷,方琦. 大蒜素对HL-60细胞端粒酶活性的影响[J]. 贵州医药, 2004, 28(4): 303-305 |
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| 作者姓名： | 王旭光 陈根殷 方琦 |
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| 作者单位： | 广东医学院附属医院,524001;广东医学院附属医院,524001;广东医学院附属医院,524001 |
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| 基金项目： | 广东省卫生厅资助(A2002498) |
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| 摘 要： | 目的 研究大蒜素对人早幼白血病HL-60细胞端粒酶活性的影响。方法 不同浓度的大蒜素作用HL-60细胞后，MTT法测细胞的生长和增殖情况，TRAP-PCR-ELISA法研究细胞端粒酶活性的变化。结果 大蒜素能明显地抑制HL-60细胞的生长，且呈时间、浓度依赖性。不同浓度的大蒜素能下调HL-60细胞的端粒酶活性，且同样呈时间、浓度依赖性。结论 大蒜素可抑制HL-60细胞的端粒酶活性，可能是其诱导HL-60细胞凋亡的重要机制之一。
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| 关 键 词： | 大蒜素 端粒酶 HL-60细胞 |
Effect of allicin on telomerase of HL-60 cell |
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| Wang Xuguang,Chen Genyin,Fang Qi,The stAffiliated Hospital of Guangdong Medical College. Effect of allicin on telomerase of HL-60 cell[J]. Guizhou Medical Journal, 2004, 28(4): 303-305 |
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| Authors: | Wang Xuguang Chen Genyin Fang Qi The stAffiliated Hospital of Guangdong Medical College |
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| Affiliation: | Wang Xuguang,Chen Genyin,Fang Qi,The 1stAffiliated Hospital of Guangdong Medical College,524001 |
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| Abstract: | Objective To investigate the effect on telemerase of HL-60 cells treated by allicin. Methods HL-60 cells were treated by allicin with different concentrations. Then the inhibiting effect and the change of telomerase activity in HL-60 cells were detected by MTT assay and TRAP-PCR-ELISA. Results The proliferation of HL-60 cells treated with allicin was significantly inhibited, and it depended on density and time. Telomerase activity of HL-60 cells treated with allicin in different concentrations was significantly inhibited, and the effect also depended density and time. Conclusions Allicin can inhibit the telomerase activity of HL-60 cells. It may be one of important regulatory mechanisms in the apoptosis of HL-60 cell. |
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| Keywords: | Allicin Telomerase HL-60 cell |
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