骨关节炎与免疫关联性初探

为了探讨免疫因素是否介导骨关节炎的发病 ,应用直接免疫荧光法检测了 6例晚期骨关节炎患者滑膜液中浸润淋巴细胞分化抗原的表达 ,同时用微量酶联免疫技术测定了这些患者滑膜液中IL 10、IL 12和sFasL的含量。结果显示关节滑膜液中的淋巴细胞以CD8+ 细胞为主 (6 7 0 %± 14 6 %) ,远远高于CD4+ 细胞 (15 0 %± 12 8%) ,两者相差显著 (P <0 0 1)。CD4+ /CD8+ 比值 <1。进一步分析表明其T细胞受体主要为αβ型TCR (6 4 0 %± 12 6 %)。滑膜液中IL 12含量 (2 0 3 84±49 2 )pg/ml高于IL 10 (37 8± 14 5 )pg/ml,有明显统计学差异 (P <0 0 1)。外周血中IL 10和IL 12的含量分别为 (4 3 37±14 2 )pg/ml和 (4 5 5 8± 10 6 )pg/ml。血清中sFasL含量较高 (15 3 90± 5 6 )pg/ml,而滑膜液中则很低 (<5pg/ml,P <0 0 1)。提示在骨关节炎的晚期CD8+ 淋巴细胞和IL 12增高可能参与了关节的免疫损伤。

A Prilimiary Study on Some is Immunopathological Features in the Joint Lesion of Osteoarthritis

To explore whether immunuopathological mechanism is connected with the joint lesion of osterorthritis(OA),synovium-lymphocytes were phenotypically analysed using methed of immunofluroscence for 6 patients with OA.The concentration of IL-10,IL-12 and sFasl in synovium were also determined with ELISA.The data we obtained showed that in all of the patients,most of the T cells in synovium were CD8 positive(67.0%±14.6%)while the percent of CD4 positive cells was (15.0%±12.8%).Meanwhile,the concentration of IL-12 in the synovium was much higher(203.84±49.2)pg/ml than that of IL-10 (37.8±14.5)pg/ml (P<0.05).The level of IL-10 and IL-12 in serum from same patients were lower,(43.37±14.2)pg/ml and (45.58±10.6)pg/ml respectively.In contrast,the concentration of the sFasL was very low in the synovium fluid but reached high level in serum(153.90±56)pg/ml.Our data strongly indicated that there were T cells in the synovium fluid.The imbalance of IL-12 and IL-10 may involved in the pathogenesis of OA.