当归及其多糖对隐孢子虫感染小鼠的免疫调节作用

目的 探讨当归及当归多糖对隐孢子虫感染小鼠的免疫调节作用.方法 连续8 d灌服给小鼠醋酸地赛米松后,于第9 d经口接种1×10^6个隐孢子虫卵囊1次,建立隐孢子虫感染模型,再经当归水煎剂及当归多糖灌胃治疗10 d,计数粪便隐孢子虫卵囊,观察肠组织病理学改变,检测T细胞亚群和血清IL-2、IL-4、IFN-γ水平.并设正常对照、模型对照和西药对照组.结果 与模型组比较,西药组、当归水煎剂组和当归多糖组小鼠卵囊排除数量减少（第4天分别为300个、50个、238个和230个）,肠组织病理学改变较轻,免疫学检测发现CD4^＋T细胞分别为（38.85±8.16）%、（47.98±11.90）%、（55.85±6.63）%、（51.43±8.42）%]CD4^＋/CD8^＋分别为1.98±0.47、2.25±0.53、2.51±0.55、2.42±0.23）]及IL-2的水平分别为（28.75 4±1.93）pg/ml、（69.02±10.47）ps/ml、（42.91±4.48）pg/ml、（40.90±0.79）pg/ml]IL-4的水平分别为（42.00±6.79）pg/ml、（64.26±6.07）pg/ml、（58.31±9.13）ps/ml、（54.95±8.99）pg/ml]和IFN-γ的水平分别为（28.73±8.71）ps/ml、（45.40±6.11）pg/ml、（84.40±7.63）pg/ml、（78.40±6.32）pg/ml]明显升高（F值分别为13.58、19.37、24.22、54.36和35.74 P值均小于0.05）.结论 当归水煎剂和当归多糖通过增强机体免疫功能促进隐孢子虫感染的免疫抑制小鼠的恢复.

Immune regulation of Angelica and its polysaccharide on the cryptosporidium-infected immunosuppressed mice

Objective To investigate the immune regulation of Angelica decoction and Angelica polysaccharide on the immunosuppressed mice infected with cryptosporidium. Methods Model mice were established by oral inoculation of 1 ×106 cryptosporidium oocysts immediuafely after being fed with betamethasone acetate for 8 days. Then the model mice were orally treated with Angelica decoction and its polysaccharide for 10 days. Cryptosporidium oocysts in faeces were daily counted. Intestinal histopathological changes, T cell subsets, and levels of serum IL-2, IL-4,and IFN-γ were detected, meanwhile normal control, model control and western medicine control were set. Results Compared with model group, decreased excluded oocysts (At the fourth day, they are 300, 50, 238, 230) , less intestinal histopathological changes, higher immunological CD4+ T cells (Model group, western medicine group, Angelica decoction group and its polysaccharide group are 38.85 8.16, 47.98 11.90, 55.85 6. 63, 51.43 8.42 respectively. ), CD4+/CD8+(1.98 0.47, 2.25 0.53, 2.51 0.55, 2.42 0.23 respectively) and IL-2 (28.75 1.93) pg/ml, (69.02 10.47) pg/ml, (42.91 4.48) pg/ml, (40.90 0.79) pg/ml respectively], IL-4 (42. 00 6. 79) pg/ml, (64.26 6.07) pg/ml, (58.31 9.13) pg/ml, (54.95 8.99) pg/ml respectively] and IFN-γ (28.73 8.71) pg/ml, (45.40 6. 11) pg/ml, (84.40 7.63) pg/ml, (78.40 6.32) pg/ml respectively] levels were found in the mice of western medicine group, Angelica decoction group and its polysaccharide group (Value F arel3.58, 19.37, 24.22, 54.36 and 35.74. Value P are all smaller than 0.05). Conclusion Angelica decoction and its polysaccharide can promote the mice infected with cryptosporidium to resume by enhancing the immune function.