HPLC法测定清热地黄酊中毛蕊花糖苷的含量

目的:建立测定清热地黄酊中毛蕊花糖苷含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Symmetry Shield C18(250 mm×4.6 mm,5μl),流动相为乙腈-0.1%醋酸溶液(10∶90,V/V),检测波长为334 nm,流速为1.0 ml/min,柱温为30△,进样量为10μl。结果:毛蕊花糖苷质量浓度在187.6⁹38.0μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 7);精密度、稳定性、重复性试验的RSD均≤0.52%;平均加样回收率为99.97%,RSD=0.28%(n=9)。结论:该方法简单、准确、重复性好,可用于清热地黄酊中毛蕊花糖苷的含量测定。

Content Determination of Verbascoside in Qingre Dihuang Tincture

OBJECTIVE：To establish the method for the content determination of verbascoside in Qingre dihuang tincture.METHODS：HPLC method was adopted. The determination was performed on Waters Symmetry Shield C18（250 mm×4.6 mm,5 μl）column with mobile phase consisted of acetonitrile-0.1% acetum solution（10∶90,V/V）at the flow rate of 1.0 ml/min. The detection wavelength was set at 334 nm and the column temperature was 30 ℃. The sample size was 10 μl. RESULTS：The linear range of verbascoside was 187.6-938.0 μg/ml（r=0.999 7）. RSDs of precision,stability and reproducibility tests were all lower than0.52% with an average recovery of 99.97%（RSD=0.28%,n=9）. CONCLUSIONS：The method is simple,accurate and reproducible,and can be used for content determination of verbascoside in Qingre dihuang tincture.