肝细胞癌细胞膜抗原性的初步研究

为探讨人肝癌细胞膜是否存在与癌周肝细胞膜不同的抗原表达,作者用4株人肝癌体外培养细胞分别免疫家兔,抗血清经肝粉吸附后对外科切除的33例肝细胞癌及癌周组织,10例非癌性肝病组织的冰冻切片进行了间接免疫荧光及PAP染色。结果肝癌细胞膜染色阳性,胞浆、胞核阴性;癌周组织及非癌性肝病组织中肝细胞膜也呈阴性。部分癌及非癌组织其血窦壁显弱阳性。作定量分析的结果表明,在同一张切片中,肝癌组织染色强度为癌周组织的2～17倍。结果提示肝癌细胞膜上有癌周肝细胞膜不表达或表达甚微的抗原存在。

A PRELIMINARY STUDY ON CELL MEMBRANE ANTIGENICITY IN HEPATOCELLULAR CARCINOMA

To determine whether there are different membranous antigem expressions among primary human hepatocellular carcinoma cells and hepatocytes, four intact human hepatoma cell lines namely BEL-7402, QGY-7703, MAH, SMMC-7721 as well as Hela cells were used to immunize New Zealand white rabbits. Antisera obtained were adsorbed on powdered human liver. Frozen sections from hepatocellular carcinoma (HCO) (33 cases), and non-cancerous liver tissue (10 cases) were stained by indirect immunofluorescence and PAP techniques. The incidence of positive membranous staining of cancer cells among 33 cases of HCC using various antisera wero as follows: anti-BEL-7402, 78.8%; anti-QGY-7703, 69.7%; anti-MAH, 48.5,%; anti-SMMC-7721 and anti-Hela, 15.2%; but the cytoplasm and nuclei of HCC wore negative. The above membranous staining, of antisera treated jiver tissue and hepatocytes surrounding HCC or non-HCC hepatocytes were negative. In some cases of HCC and non-HCC liver tissues, weak staining of the sinus wall could be seen. Using the microspectrometer, 8 kinds of antisera treated sections of HCC and liver tissue using PAP staining technique were scanned for comparing the intensity of staining quantitatively in the same sections. The intensity was 2-17 times higher in HCG than in the liver tissues surrounding HCC. These findings suggest that there are different membranous antigen expressions between HCC and the surrounding liver cells.