丙型肝炎病毒核心基因免疫诱生细胞免疫应答研究

目的 研究丙型肝炎病毒（ＨＣＶ）核心（Ｃ）基因免疫在诱生特异性细胞免疫应答中的作用。方法 将包含ＨＣＶ Ｃ基因片段的重组真核表达质粒ｐｃＣＮＡ ＨＣＶ Ｃ,在主宰其可以在小鼠骨髓瘤ＳＰ２／０（Ｈ－２^d）中表达之后,注射ＢＡＬＢ／ｃ小鼠股四头肌。ＥＬＩＳＡ法检测血清中抗体水平;^3Ｈ－ＴｄＲ掺入法测定免疫小鼠脾细胞特异性增殖能力;^51Ｃｒ释放法检测免疫小鼠细胞毒性Ｔ淋巴细胞（ＣＴＬｓ）体外杀伤功能。结

Immune responses to recombinant expression plasmid encoding hepatitis C virus core antigen

OBJECTIVE: To study the feasibility of DNA immunization with recombinant expression plasmid containing hepatitis C virus (HCV) core gene against HCV infection. METHODS: The recombinant plasmid containing HCV C gene was constructed and expressed transiently by using lipofectamine in the mouse SP2/0 cells. After appraisal and purification, these plasmid and control DNA were directly injected intramuscularly into BALB/c mice, HCV C specific antibody in the sera was checked with ELISA. Proliferative responses of spleen cells from experimental and control mice were determined by (3)H thymidine incorporation and cytotoxicity T lymphocyte (CTL) activity was measured by standard 4-h (51)Cr release assays. RESULTS: All immunized mice developed anti-HCV core antibodies and this antibody was dose and immune number dependent. Lymphoproliferative responses for HCV core antigen were higher in immunized mice than in control mice (P < 0.05), stimulation indices (SI) were 2.42 - 6.10. CTLs might induce approximately 63.13% lysis of pcDNA HCV C transfected SP2/0 target cells. CONCLUSION: It is suggested that HCV C gene immunization is a promising method of vaccination against HCV infection.