格林-巴利综合征与空肠弯曲菌Penner血清型的关系

目的 探索格林－巴利综合征（ＧＢＳ）的发病机制与空肠弯曲菌（ＧＪ）的关系以及我国ＧＢＳ病前ＣＪ感染率比较高的原因。方法 用改良的Ｓｋｉｒｒｏｗ方法在１９９５～１９９７年收集的４７例ＧＢＳ患者的粪便标本中分离ＣＪ,以Ｐｅｎｎｅｒ方法进行血清学分型,对不能分离出ＣＪ的患者粪便标本进行聚合酶反应（ＰＣＲ）－ＲＦＬＰ分型。对同期１７１例ＣＪ肠炎患者的ＣＪ菌株用Ｐｅｎｎｒ方法进行血清学分型。结果 共分离出６

The relationship between Guillain-Barre syndrome and serotypes of Campylobacter jejuni isolated from the patients

Objective To investigate the relationship between Campylobacter jejuni (CJ) serotype and the high incidence of Guillain Barre syndrome (GBS) following CJ enteritis in China. Methods CJ was isolated from fresh stool of 47 GBS patients in Beijing area from 1995 1997 with a combination of Skirrow method and micro filtration method. Serotpying of the CJ strains was performed with passive hemagglutination method with standard serum from CDC. 171 CJ strains from CJ enteritis in our hospital in the same period were serotyped with the same method. The stool from which no CJ could be isolated was typed with PCR RFLP method. Results 3 Penner 2, 1 Penner 4, 1 Penner 19 and 1 Penner 26 strains were isolated and serotyped. Three strains which could not be isolated were found by PCR RFLP method to be DNA I, which is equivalent to the type of Penner 19. There were some CJ strains which could be typed with serum from CDC. Conclusion CJ from GBS patients in Beijing area are similar to those reported in the world; it is suggested that CJ may cause GBS by molecular mimicry mechanism in China. The most common serotype of CJ from GBS was reported to be Penner 19 in Japan, while it was rare in CJ enteritis. However, the similarity of the serotypes between CJ from GBS patients and CJ from enteritis patients may explain the high incidence of GBS following CJ infection in China. Penner 26 is reported to be isolated from GBS patients for the first time.