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| 沉默长链非编码RNA POU5F1B抑制食管癌细胞增殖、侵袭和迁移 | |
| 引用本文： | 孟 碧1,邢 瑶1,高 飞1,王梦洁2,李 皓1,叶 婷1,刘阳晨1. 沉默长链非编码RNA POU5F1B抑制食管癌细胞增殖、侵袭和迁移[J]. 现代肿瘤医学, 2019, 0(14): 2445-2448. DOI: 10.3969/j.issn.1672-4992.2019.14.005 |
| 作者姓名： | 孟 碧1 邢 瑶1 高 飞1 王梦洁2 李 皓1 叶 婷1 刘阳晨1 |
| 作者单位： | 1.蚌埠医学院附属泰兴市人民医院，江苏 泰兴 225400;2.安徽省胸科医院，安徽 合肥 230000 |
| 基金项目： | National Natural Science Foundation of China(No.81702377);国家自然科学基金(编号:81702377)；泰兴市人民医院院课题(编号:try1728);蚌埠医学院研究生科研创新计划(编号:Byycx1750) |
| 摘 要： | 目的:探讨长链非编码RNA POU5F1B在食管癌细胞中的表达及临床意义。方法:采用实时定量PCR法比较POU5F1B在食管癌患者血浆和正常人血浆中的表达，检测POU5F1B在细胞系中的表达。敲除POU5F1B后，采用CCK-8和克隆形成试验评价沉默POU5F1B对细胞增殖的影响。采用Transwell和划痕实验检测POU5F1B对食管癌细胞侵袭和迁移的影响。结果:本研究中，我们发现POU5F1B在食管癌患者血浆中和细胞系中高表达。si-POU5F1B在体外能显著降低细胞增殖、侵袭和迁移能力。结论:本研究表明POU5F1B在食管癌中充当癌基因作用，为抗食管癌治疗提供了新的策略。 |
| 关 键 词： | 长链非编码RNA POU5F1B 食管癌 |
Silencing long non-coding RNA POU5F1B inhibits the proliferation,invasion and migration of esophageal cancer cells |
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| Meng Bi1,Xing Yao1,Gao Fei1,Wang Mengjie2,Li Hao1,Ye Ting1,Liu Yangchen1. Silencing long non-coding RNA POU5F1B inhibits the proliferation,invasion and migration of esophageal cancer cells[J]. Journal of Modern Oncology, 2019, 0(14): 2445-2448. DOI: 10.3969/j.issn.1672-4992.2019.14.005 | |
| Authors: | Meng Bi1 Xing Yao1 Gao Fei1 Wang Mengjie2 Li Hao1 Ye Ting1 Liu Yangchen1 |
| Affiliation: | 1.Taixing People＇s Hospital Affiliated to Bengbu Medical College，Jiangsu Taixing 225400，China;2.Anhui Provincial Chest Hospital，Anhui Hefei 230000,China. |
| Abstract: | Objective:To investigate the expression and clinical significance of long non-coding RNA in human esophageal carcinoma cells.Methods:The PCR method was used to compare the expression of POU5F1B in EC plasma and normal human plasma.After the knockout of POU5F1B,CCK-8 and clonal formation experiments were used to evaluate the effect of silent POU5F1B on cell line proliferation.The effects of POU5F1B on invasion and migration of EC cell lines were detected by Transwell and scratch test.Results:In this study,we found that POU5F1B was highly expressed in plasma and cell lines of esophageal cancer patients.Si-POU5F1B significantly reduced cell proliferation,invasion and migration in vitro.Conclusion:POU5F1B acts as an oncogene in esophageal cancer and provindes a new strategy for the treatment of esophageal cancer. |
| Keywords: | lncRNA POU5F1B esophageal cancer |
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