| 硼替佐米对胰腺癌细胞增殖、凋亡及PI3K/Akt通路的影响 |
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| 引用本文： | 白晓黎1,范 华1,陈宏伟2. 硼替佐米对胰腺癌细胞增殖、凋亡及PI3K/Akt通路的影响[J]. 现代肿瘤医学, 2019, 0(24): 4323-4328. DOI: 10.3969/j.issn.1672-4992.2019.24.001 |
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| 作者姓名： | 白晓黎1 范 华1 陈宏伟2 |
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| 作者单位： | 1.河南科技大学第一附属医院药剂科,河南 洛阳 471000；2.郑州大学附属洛阳中心医院消化内科,河南 洛阳 471000 |
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| 基金项目： | National Natural Science Foundation of China(No.U1504808);国家自然科学基金资助项目（编号:U1504808） |
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| 摘 要： | 目的:探讨硼替佐米（BTZ）对胰腺癌细胞增殖、凋亡及PI3K/Akt通路的影响。方法:体外培养人胰腺癌细胞株PANC-1细胞，分别给予硼替佐米（0，2，10，50，250 nmol/L）作用24 h，采用MTT法检测PANC-1细胞活力，采用Annexin V/PI双染法检测细胞凋亡情况，采用流式细胞术检测细胞周期，采用Western blot检测细胞凋亡及PI3K/Akt通路相关蛋白表达情况。结果:与0 nmol/L硼替佐米相比，不同浓度硼替佐米处理后，PANC-1细胞生长抑制率均显著升高（P＜0.05），G0/G1期细胞比例均显著升高（P＜0.05），细胞凋亡率显著升高（P＜0.05），细胞中cleaved-caspase-3、Bax蛋白表达增高（P＜0.05），Bcl-2、PI3K、p-Akt蛋白表达降低（P＜0.05），且呈剂量依赖性；裸鼠成瘤实验中，不同浓度硼替佐米作用后，肿瘤瘤体质量显著降低（P＜0.05），且呈剂量依赖性。结论:硼替佐米能抑制胰腺癌细胞PANC-1增殖，诱导其凋亡，推测硼替佐米可能通过调节PI3K/Akt通路，激活凋亡蛋白基因表达，抑制抗凋亡蛋白基因的表达，诱导PANC-1细胞凋亡。
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| 关 键 词： | 胰腺癌 硼替佐米 细胞增殖 细胞凋亡 PI3K/Akt通路 |
The effects of bortezomib on proliferation,apoptosis and PI3K/Akt pathway of pancreatic cancer cells |
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| Bai Xiaoli1,Fan Hua1,Chen Hongwei2. The effects of bortezomib on proliferation,apoptosis and PI3K/Akt pathway of pancreatic cancer cells[J]. Journal of Modern Oncology, 2019, 0(24): 4323-4328. DOI: 10.3969/j.issn.1672-4992.2019.24.001 |
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| Authors: | Bai Xiaoli1 Fan Hua1 Chen Hongwei2 |
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| Affiliation: | 1.Department of Pharmacy,the First Affiliated Hospital of Henan University of Science and Technology,Henan Luoyang 471000,China;2.Department of Gastroenterology,Zhengzhou University Affiliated Luoyang Central Hospital,Henan Luoyang 471000,China. |
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| Abstract: | Objective:To investigate the effects of bortezomib (BTZ) on proliferation,apoptosis,and PI3K/Akt pathway of pancreatic cancer cells.Methods:Human pancreatic cancer cell line PANC-1 cells were cultured in vitro.They were given bortezomib treatment (0,2,10,50,250 nmol/L) respectively for 24 h.MTT assay was used to detect the viability of PANC-1 cells.Annexin V/PI double staining was used to detect apoptosis.Cell cycle was detected by flow cytometry.Western blot was used to detect apoptosis and PI3K/Akt pathway related protein expression.Results:Compared with 0 nmol/L BTZ,after treatment with bortezomib at different concentrations,the growth inhibition rate of PANC-1 cells increased significantly (P＜0.05),the proportion of G0/G1 phase cells increased significantly (P＜0.05),the apoptosis rate increased significantly (P＜0.05),the expressions of cleaved-caspase-3 and Bax protein in the cells increased (P＜0.05),the protein expressions of Bcl-2,PI3K,and p-Akt decreased (P＜0.05),in dose-dependent manners.In the tumor-forming experiment of nude mice,the tumor mass was significantly decreased (P＜0.05) in dose-dependent manners.Conclusion:Bortezomib can inhibit the proliferation and induce apoptosis of pancreatic cancer cell PANC-1.It is speculated that bortezomib may activate apoptotic protein gene expression,inhibit anti-apoptotic protein gene expression,and induce PANC-1 cell apoptosis by regulating PI3K/Akt pathway. |
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| Keywords: | pancreatic cancer bortezomib cell proliferation apoptosis PI3K/Akt pathway |
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