PKM2表达和巨噬细胞中CD163升高协同促进骨肉瘤进展

目的 探讨骨肉瘤中PKM2与相关病理学特征的关系，以进一步寻找判断骨肉瘤预后的标志物。方法 收集原发性骨肉瘤石蜡包埋标本88例，并对标本进行组织微阵列构建，免疫组织化学法检测PKM2的表达情况， Kaplan-Meier分析预后价值，Cox回归模型进行参数与患者预后的相关性分析。结果 M2巨噬细胞特异性标志物CD163在骨肉瘤基质中升高，PKM2过表达与M2巨噬细胞浸润在骨肉瘤中呈正相关。结论 PKM2过表达和M2巨噬细胞浸润是骨肉瘤患者预后不良的因素。

PKM2 Expression and Elevated CD163 in Macrophage Synergistically Promote Osteosarcoma Progression

Objective To investigate the relation between PKM2 and related pathological features of osteosarcoma, to further explore the markers for predicting the prognosis of osteosarcoma patients. Methods We collected 88 cases of paraffin-embedded primary osteosarcoma specimens, and the specimens were constructed by tissue microarray. Serial sections were used to detect the expression of PKM2 in the above tissues by immunohistochemistry. Kaplan-Meier analysis was used to demonstrate the prognostic value. Correlation analysis between parameters and patient’s prognosis was performed using Cox regression model. Results M2 macrophage-specific marker CD163 was elevated in osteosarcoma matrix, suggesting that M2 macrophages were densely infiltrated in osteosarcoma. PKM2 overexpression is positively correlated with M2 macrophage infiltration in osteosarcoma. Conclusion PKM2 overexpression and M2 macrophage infiltration were influence factors for patients’ poor prognosis.