miR-139-5p靶向GPR56抑制骨肉瘤细胞增殖、迁移和侵袭的实验研究

目的:研究miR-139-5p对骨肉瘤细胞增殖、迁移和侵袭的影响，并探讨其机制。方法:运用qRT-PCR检测软骨肉瘤细胞中miR-139-5p、GPR56的mRNA表达；Western blot检测细胞中GPR56的蛋白表达；将miR-139-5p组（转染miR-139-5p mimics）、miR-NC组（转染miR-NC）、si-NC组（转染si-NC）、si-GPR56组（转染si-GPR56）、miR-139-5p+pcDNA3.1组（miR-139-5p mimics和pcDNA3.1共转染）、miR-139-5p+pcDNA3.1-GPR56组（miR-139-5p mimics和pcDNA3.1-GPR56共转染），均以脂质体法转染至U-2OS细胞；MTT法检测各组细胞的增殖；Transwell检测各组细胞的迁移、侵袭。结果:与人正常成骨细胞hFOB1.19相比，人骨肉瘤细胞U-2OS中miR-139-5p表达显著降低，GPR56表达显著升高（P＜0.05）。过表达miR-139-5p、敲减GPR56均可明显抑制U-2OS细胞增殖、迁移、侵袭；GPR56是miR-139-5p的靶点。过表达GPR56可逆转miR-139-5p对U-2OS细胞增殖、迁移、侵袭的抑制作用。结论:miR-139-5p可抑制骨肉瘤细胞增殖、迁移、侵袭，其机制可能与靶向GPR56有关，将可为骨肉瘤的治疗提供新靶点。

Experimental study of miR-139-5p inhibiting proliferation,migration and invasion of osteosarcoma cells by targeting GPR56

Objective:To study the effects of miR-139-5p on proliferation,migration and invasion of osteosarcoma cells and explore its mechanism.Methods:qRT-PCR was used to detect the mRNA expression of miR-139-5p and GPR56 in chondrosarcoma cells.Western blot was used to detect the protein expression of GPR56 in cells.The miR-139-5p group (transfected miR-139-5p mimics),miR-NC group (transfected miR-NC),si-NC group (transfected si-NC),si-GPR56 group (transfected si-GPR56),miR-139-5p+pcDNA3.1 group (co-transfected miR-139-5p mimics and pcDNA3.1),miR-139-5p+pcDNA3.1-GPR56 (co-transfected miR-139-5p mimics and pcDNA3.1-GPR56),all transfected to U-2OS cells by liposome method.MTT assay was used to detect the proliferation of each group.Transwell was used to detect the migration and invasion of each group.Results:Compared with human normal osteoblasts hFOB1.19,the expression of miR-139-5p was significantly decreased and the expression of GPR56 was significantly increased in human osteosarcoma cell U-2OS (P＜0.05).Overexpression of miR-139-5p and knockdown of GPR56 significantly inhibited proliferation,migration and invasion of U-2OS cells.GPR56 was a target of miR-139-5p.Overexpression of GPR56 reversed the inhibitory effect of miR-139-5p on proliferation,migration and invasion of U-2OS cells.Conclusion:miR-139-5p can inhibit the proliferation,migration and invasion of osteosarcoma cells.The mechanism may be related to targeting GPR56,which will provide a new target for the treatment of osteosarcoma.