miR-152通过调节AKT-ERK信号通路对骨肉瘤细胞增殖和迁移的影响

目的:探讨miR-152对骨肉瘤细胞增殖和迁移的影响。方法:通过实时定量PCR法检测miR-152在骨肉瘤组织和细胞中的表达；在骨肉瘤细胞中进行miR-152过表达后，MTT联合克隆形成法检测细胞增殖的变化；划痕法考察miR-152对骨肉瘤细胞迁移的影响；Western blot法检测骨肉瘤细胞中AKT-ERK的表达；免疫组化方法分析骨肉瘤组织和癌旁组织中AKT-ERK蛋白的表达。结果:相比于癌旁组织以及成骨细胞，miR-152在人骨肉瘤组织和骨肉瘤细胞中表达有不同程度降低，其中在U2OS细胞中降低最为显著（P<0.01）；在U2OS细胞中过表达miR-152，72 h后细胞增殖能力明显降低（P<0.01）；同时，骨肉瘤细胞的迁移率也显著降低（P<0.01）。AKT-ERK蛋白在骨肉瘤组织和细胞中高表达，miR-152能够降低AKT-ERK表达及其磷酸化。结论:miR-152作为一种抑癌小RNA，其能够通过调节AKT-ERK通路，从而抑制骨肉瘤细胞的增殖、迁移。

Effects of miR-152 on proliferation and migration of osteosarcoma cells by regulating AKT-ERK signaling pathway

Objective:To investigate the effects of miR-152 on cell proliferation and migration in osteosarcoma (OS) cells.Methods:The level of miR-152 in osteosarcoma tumors and cells was evaluated by RT-qPCR.After miR-152 overexpression in OS cells，MTT combined with colony survival assay were applied to measure the proliferation ability of the cells.Furthermore，wound healing assay was used to determine the migration ratio of the OS cells.Western blot assay was used to analyze the expression of AKT-ERK in OS cells.Immunohistochemical assay was applied to measure the protein expression of AKT-ERK in OS tissues.Results:Compared with peritumor and osteoblast cells，the level of miR-152 in OS tissues and cells was decreased，especially in U2OS cells (P<0.01).Under the overexpression of miR-152 in U2OS cells，the proliferation of the cells was significantly decreased after 72 h of transfection (P<0.01) and the cell migration was also significantly decreased compared with control group (P<0.01).The expression of AKT-ERK was higher in OS tumors and cells and miR-152 could inhibit the AKT-ERK signal pathway in OS cells.Conclusion:As a tumor suppressor miRNA，miR-152 could inhibit the proliferation and migration of OS cells via regulation of AKT-ERK signal pathway.