细丝蛋白A在宫颈癌患者癌组织中的表达及其临床意义

目的：探讨宫颈癌组织中细丝蛋白A （FLNa）的表达及其与宫颈癌患者临床病理学特征的关系,分析FLNa表达与判断预后指标的关联性,阐明FLNa作为评价宫颈癌预后指标的可行性,为临床早期评估宫颈癌预后提供依据。方法：采用免疫组织化学法检测55例宫颈癌组织和癌旁组织中FLNa阳性表达率,RT-PCR法检测20例宫颈癌组织和癌旁组织中FLNa mRNA表达水平,分析FLNa表达与宫颈癌患者临床病理参数的关系。结果：免疫组织化学检测,FLNa定位于细胞质,基底细胞中FLNa表达阳性率高,细胞间质也有部分表达。FLNa在宫颈癌组织中高表达,在癌旁正常组织中不表达。Ⅰ+Ⅱ期宫颈癌患者宫颈癌组织中FLNa阳性表达率低于Ⅲ+Ⅳ期患者（P<0.05）。宫颈癌患者癌组织中FLNa表达与患者TNM分期、淋巴结转移和子宫旁浸润有关联（P<0.05）。RT-PCR检测,在20例宫颈癌组织中FLNa mRNA表达水平高于癌旁正常组织（P<0.05）。结论：FLNa可能成为1项评估宫颈癌患者预后的指标。

Expression of filamin A in cancer tissue of cervical cancer patients and its clinical significance

Objective: To investigate the expression of filamin A (FLNa) in cervical cancer tissue and its relationships with the clinicopathological characteristics of the cervical cancer patients, to analyze the correlations between the expression of FLNa and the prognostic indicators, to clarify the feasibility of FLNa as a prognostic indicator for cervical cancer,and to provide the evidence for early clinical evaluation on the prognosis of cervical cancer. Methods: The positive expression rates of FLNa in 55 cases of cervical cancer tissue and adjacent tissue were detected by immunohistochemical method,the expression levels of FLNa mRNA in 20 cases of cervical cancer and adjacent tissues were detected by RT-PCR method, and the relationships between the expression of FLNa and the clinicopathological parameters of the patients with cervical cancer were analyzed. Results: The immunohistochemical results showed that FLNa was localized in the cytoplasm,the positive expression rate of FLNa in the basal cells was high and it expressed partly in the interstitium.FLNa highly expressed in the cervical cancer tissue and did not express in the adjacent normal tissue.The positive expression rate of FLNa in the patients with stage Ⅰ+Ⅱ cervical cancer was lower than that in the patients with stage Ⅲ+Ⅳ cervical cancer (P<0.05).The expression of FLNa in cancer tissue of the patients with cervical cancer was associated with TNM stage,lymph node metastasis and parametrial invasion (P<0.05).The RT-PCR results showed that the expression level of FLNa in 20 cases of cervical cancer tissue was higher than that in adjacent normal tissue (P<0.05). Conclusion: FLNa may become a indicator of evaluation on the prognosis of the patients with cervical cancer.