| 两株犬源狂犬病病毒街毒株全基因组序列测定与分析 |
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| 引用本文： | 齐瑛琳,金宏丽,赵丽丽,王化磊,赵平森,付玉,涂长春,杨松涛,夏咸柱. 两株犬源狂犬病病毒街毒株全基因组序列测定与分析[J]. 中国病原生物学杂志, 2011, 0(7) |
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| 作者姓名： | 齐瑛琳 金宏丽 赵丽丽 王化磊 赵平森 付玉 涂长春 杨松涛 夏咸柱 |
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| 作者单位： | 吉林大学农学部畜牧兽医学院;军事医学科学院军事兽医研究所;中国医学科学院医学实验动物研究所; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(No.2011CB504706); 国家科技重大项目(No.2009ZX10602-14) |
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| 摘 要： | 目的对2株犬源狂犬病病毒街毒株全基因组测序,了解国内狂犬病病毒的流行和变异情况。方法采用乳鼠脑内接种方法分离2株天津地区狂犬病病毒街毒株,RT-PCR扩增病毒DNA覆盖全基因组12个相互重叠的基因片段,PCR产物平端克隆后进行全基因组核苷酸序列测定,然后对其分子变异和进化特点进行分析。进行两分离株的序列相似性、氨基酸同源性、主要功能位点的变异比较和种系发生分析。结果 TJD04和TJD05两株狂犬病病毒全基因组长均为11 924 nts,全基因组序列的组成和结构均符合弹状病毒科狂犬病病毒属的特征。病毒基因组由5个编码区组成,基因起始位点和终止位点高度保守,氨基酸编码长度未发生变异,5个编码蛋白序列较少发生变化,仅有个别主要功能位点发生变异,且大部分变异均属于无意义沉默突变。多序列相似性比较和种系发生分析显示,两株病毒均属于基因1型,N基因与全基因进化树保持一致,与中国犬源狂犬病病毒BD06同源性最高(99.6%),进化关系近,并具有较为独特的中国地域特点。结论两天津株狂犬病毒可能是自然界中固有的狂犬病病毒街毒株。
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| 关 键 词： | 狂犬病病毒 街毒株 全基因组 序列分析 |
Sequencing and analysis of the complete genome of two wild-type strains of rabies virus isolated from dogs |
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| QI Ying-lin,,JIN Hong-li,ZHAO Li-li,WANG Hua-lei,ZHAO Ping-sen,,FU Yu,TU Chang-chun,YANG Song-tao,XIA Xian-zhu. Sequencing and analysis of the complete genome of two wild-type strains of rabies virus isolated from dogs[J]. Journal of Pathogen Biology, 2011, 0(7) |
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| Authors: | QI Ying-lin JIN Hong-li ZHAO Li-li WANG Hua-lei ZHAO Ping-sen FU Yu TU Chang-chun YANG Song-tao XIA Xian-zhu |
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| Affiliation: | QI Ying-lin1,2,JIN Hong-li1,ZHAO Li-li1,WANG Hua-lei2,ZHAO Ping-sen2,3,FU Yu2,TU Chang-chun2,YANG Song-tao2,XIA Xian-zhu2 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130122,3.Comparative Medicine Center,Peking Union Medical College,Beijing 100021,China) |
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| Abstract: | Objective In order to ascertain the prevalence and diversity of rabies viruses in China,two wild-type strains of rabies virus were isolated by intracerebral inoculation of mouse pups with homogenate samples.Methods The genomes of two isolates were amplified by RT-PCR with series of specified primers and cloned into pEASY-Blunt vectors.The recombinant plasmids were sequenced and the obtained sequences of virus were processed and assembled with Seqman software from DNAstar.Results Analysis of the diversity of... |
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| Keywords: | Rabies virus wild-type full-length genome sequencing and analysis |
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