| PCR技术在血吸虫感染检测中的应用 |
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| 引用本文： | 秦圆方,祁贤,吴海玮. PCR技术在血吸虫感染检测中的应用[J]. 中国病原生物学杂志, 2011, 0(4) |
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| 作者姓名： | 秦圆方 祁贤 吴海玮 |
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| 作者单位： | 江苏省疾病预防控制中心急性传染病预防控制研究所;南京医科大学病原生物学系江苏省现代病原生物学重点实验室;国际健康研究中心美国罗德岛医院;布朗大学; |
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| 摘 要： | 血吸虫病目前仍是一种严重危害人类健康的寄生虫病。多聚酶链反应(PCR)是分子生物学技术检测DNA最灵敏的方法之一,在近期血吸虫病检测与诊断研究中的应用越来越多。虽然各研究所用PCR技术的检测标本、靶基因选择各异,诊断效率也不尽相同,但是PCR作为一种高特异性敏感性检测方法,与其他检测方法相比仍颇具优势。本文综述了PCR技术在血吸虫感染检测中的应用情况。
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| 关 键 词： | 血吸虫 PCR 核酸检测技术 敏感性 特异性 综述 |
Use of PCR to detect schistosome infection |
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| QIN Yuan-fang,,QI Xian,WU Hai-wei. Use of PCR to detect schistosome infection[J]. Journal of Pathogen Biology, 2011, 0(4) |
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| Authors: | QIN Yuan-fang QI Xian WU Hai-wei |
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| Affiliation: | QIN Yuan-fang1,2,QI Xian1,WU Hai-wei2,3 (1.Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China,2.Department of Pathogenic Biology,Jiangsu Province Key Lab of Modern Pathogenic Biology,3.Center for International Health Research,Rhode Island Hospital,Brown University,Providence,RI02903,USA) |
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| Abstract: | Schistosomiasis remains a serious threat to public health.Polymerase chain reaction(PCR) is one of the most sensitive molecular biological techniques of analyzing DNA and is increasingly used in studies to detect and diagnose schistosomiasis.Although PCR techniques involve different samples,various target genes,and different levels of diagnostic efficiency in studies,PCR still has a higher sensitivity and specificity than do other methods of detection.This review summarizes the use of PCR techniques to dete... |
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| Keywords: | Schistosome PCR nucleic acid detection technique sensitivity specificity review |
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