角质细胞生长因子促进大鼠胰腺导管上皮细胞体外增殖

目的 探讨不同浓度的角质细胞生长因子(KGF)对大鼠胰腺导管上皮细胞增殖的影响,寻求最佳浓度.方法 免疫细胞化学染色及RT-PCR方法鉴定SD大鼠胰腺导管上皮细胞；不同浓度的KGF刺激胰腺导管上皮细胞增殖,寻求最佳浓度.结果 大鼠胰腺导管上皮细胞表达Nestin和CK19,不表达Insulin及Glucagon;不同浓度KGF刺激胰腺导管上皮细胞2d后,细胞均有不同程度增殖,20 μg/L KGF作用2d时,细胞数为0.35±0.03,显著高于对照组的0.27±0.02(P ＜0.01).结论 KGF在本实验的剂量范围内可促进胰腺导管上皮细胞增殖,20 μg/L为促进增殖的最佳浓度.

Keratinocyte growth factor promotes proliferation of rat pancreatic ductal epithelium cells in vitro

Objective To investigate the effect of keratinocyte growth factor (KGF) at different concentrations on the proliferation of pancreatic ductal epithelial cells (PDECs). Methods The PDECs were identified by immunocytochemical stain and RT-PCR. PDECs counting was carried out at different KGF concentrations. Results Nestin and CK19 were positively expressed in PDECs by RT-PCR and immunocytochemical stain; PDECs were stimulated by different concentrations of KGF after 2 days, and PDECs number stimulated by 20 mg/L KGF was 0.35±0.03,much higher than that of the control group which was 0.27±0.02. Conclusions KGF at different concentrations can significantly stimulate the proliferation of PDECs, the optimized concentration in our tests was 20μg/L.