双黄连抑制流感病毒甲1型基因的研究

目的通过对实时荧光定量RT—PCR检测流感病毒甲1型多聚酶蛋白（PA）基因，了解烈黄连作用病毒的效应。方法采用实时荧光定量RT—PCR法标准曲线测药物抑制甲。型PA病毒基因效应。结果以实时荧光RT-PCR法，重复测7次取平均值，对照组病毒基因为3．62×10^6±0．90拷贝数／ug，试验组病毒基因为4．57×10^5±1．23拷贝数／mg。发现病毒对照组基因明显高于试验组（P〈0．001）。结论提示双黄连可抑制流感病毒甲1基因。

Study on Shuanghuanglian inhibiting effect of influenza virus A1 gene

Objective To establish a real - time fluorogenic quantitative RT-PCR method for detecting the expression of PA gene of influenza virus A1 gene and to investigate the iuhibitore effect of Shuanghuanglian. Methods Real-time fluorogenic quantitative RT- PCR （FQ-RT-PCR） was set up to examine the specific expression of test group and virus control group PA gene in virus. Results After 7 duplicate tests with the FQ-RT-PCR. method, the average level of the virus control group was 3.62 × 10^6 ±0.90 copies/μg RNA in virus and 4. 57 × 10^5±1.23 copies/μg RNA in virus test group. Virus control group was significantly higher than the test group （ P 〈 0. 001 ）. Conclusion This showe that Shuanghuanglian could inhibit PA gene expression of influenza virus A1.