汉麻果胶皂苷类成分分离及其抗真菌活性研究

目的获得汉麻果胶皂苷类成分的主要部位，进行抗真菌试验，测定其最小抑菌浓度值（M/C）。方法利用D101大孔树脂进行梯度洗脱，通过试管定性试验以及薄层色谱（TLC）法确定富集皂苷类成分洗脱部位，并采用微量液基稀释法研究其对4种常见致病真菌红色毛癣菌、须癣毛癣菌、犬小孢子菌和白色念珠菌的生物活性。结果80%浓度的洗脱物对红色毛癣菌、须毛癖菌、犬小孢子菌均具有抑制作用，其M/C值分别为16,8,2 ｜ig/mL。结论汉麻果胶皂苷类成分具有显著的抗真菌作用。

Study on Isolation of Saponins from China - Hemp Pectin and Their Anti - Fungal Activity

Objective To obtain the principal parts of the saponin constituents from China - Hemp pectin, to perform the in vitro anti - fungal experiment and to test the minimal inhibitory concentration（MIC）. Methods The gradient elution was performed by using D101 macroporous absorbing resin. The elution part of enriched saponins was determined by the test tube qualitative test and TLC,and the anti - fungal activities of the saponins of China - hemp pectin against trichophyton rubrum,trichophyton mentagrophytes,microsporum canis and Candida albicans were studied by the broth microdilution method. Results The China - hemp pectin eluate with the concentration of 80% had the inhibiting effect on trichophyton rubrum,trichophyton mentagrophytes,microsporum canis and their MIC values were 16,8,2 jxg/mL respectively. Conclusion The saponins of China - hemp pectin has significant anti - fungal effect.