不同采收季节的淫羊藿中朝藿定C和淫羊藿苷的含量比较

目的比较3个贵州主流产淫羊藿药材在不同采收季节中朝藿定C和淫羊藿苷的含量。方法用高效液相色谱（HPLC）法测定粗毛淫羊藿、巫山淫羊藿、黔岭淫羊藿中朝藿定c和淫羊藿苷的含量。采用EliteSinoChromODS—AP柱（250mm×4．6mm，5．0Ixm），流动相为乙腈（A）一水（B）梯度洗脱（0—22rain，27％_29％A；22～23rain，29％_÷100％A；23～34rain，100％A；34～36rain，loO％一27％A；36～50min，27％A）。结果朝藿定c和淫羊藿苷进样量分别在13．22～396．6Ixg（r=0．9999）和3．026～151．3斗g范围内与峰面积均呈良好线性关系（r=0．9999），平均回收率均在98．0％一105．O％范围内；粗毛淫羊藿中朝藿定c和淫羊藿苷的含量分别为0．516％～2．973％，0．096％～0．216％；黔岭淫羊藿中朝藿定C和淫羊藿苷的含量分别为0．071％～0．185％，0．081％～0．164％；巫山淫羊藿含朝藿定c和淫羊藿苷中朝藿定c和淫羊藿苷的含量分别为1．015％～4．219％，0．080％～0．190％。结论巫山淫羊藿中朝藿定c和淫羊藿苷的含量最高，其次为粗毛淫羊藿、黔岭淫羊藿，说明不同产地、不同品种的淫羊藿药材中朝藿定c和淫羊藿苷的含量差异较大．

Content Determination of Epimedin C and Icariin in Epimedium from Different Harvest Seasons by HPLC

Objective To compare the contents of epidemin C and icariin in 3 Guizhou mainstream varieties of the medicinal material epimedium from different harvesting season. Methods The high performance liquid chromatography（HPLC） method was adopted to de- termine the epimedin C and icariin content in Epimedium acuminatum, Epimedium wushanense T. and Epimedium leptorrhizum Steam. The samples were separated on the Elite SinoChrom ODS-AP column（250 mm×4.6 mm, 5.0 μm）;the mobile phase was acetonitrile （A） -water（B） by the gradient elution （0-22 min, 27%→29% A;22-23 min, 29%→100% A;23 -34 min, 100% A;34-36 min, 100%→27% A;36-50 rain, 27% ）. Results The linear ranges of epimedin C and icariin were 13.22-396. 6 tzg（r =0. 999 9） and 3.026- 151.3 μg（r=0.999 9） respectively. The average recovery rates of epimedin C and icariin were 98.0%- 105.0% ;the con- tents of epimedin C and icariin in Epimedium acuminatum were O. 516%-2. 973% and 0.096%-0. 216% respectively. The con- tents of epimedin C and icariin in Epimedium leptorrhizum Stearn were 0.071-0.185% and 0.081%-0. 164% respectively. The contents of epimedin C and icariin in Epimedium wushanense T. were 1.015%-4.219% and 0.080%-0. 190% respectively. Conclusion Epimedium wushanense T. has the highest content of epimedin C and icariin, followed by Epimedium acuminatum and Epirnedium leptorrhizum Steam is in the end, which indicates that different producing areas and different varieties of epimedium medici- nal material have quite different contents of epimedin C and icariin.