先天免疫分子Toll-Like-Receptor-9在慢性鼻窦炎鼻息肉发病机制中的作用探讨

目的:检测先天免疫分子Toll-Like-Receptor-9(TLR-9)蛋白在鼻息肉黏膜上皮细胞中的存在及表达,以期探讨其在鼻息肉发病中的作用及功能.方法:取鼻窦内镜下摘除的鼻息肉标本10例(鼻息肉组),鼻中隔偏曲和鼻外伤等手术切除的下鼻甲标本10例作对照组,消化法行原代细胞培养,流式细胞仪双抗纯化上皮细胞和检测上皮细胞中TLR-9蛋白含量.结果:①消化法培养人鼻黏膜原代细胞中上皮占98%;②鼻息肉组和对照组上皮细胞均有TLR-9表达,但鼻息肉组的TLR-9表达(11%～15%)显著低于对照组(49%～60%).结论:TLR-9存在于正常和鼻息肉的黏膜上皮中,但鼻息肉组水平下降,推测先天免疫分子TLR-9可以在鼻息肉发病机制中起作用.

Effects of Toll-Like-Receptor-9 expression in chronic rhinosinusitis with nasal polyps

Objective:To determine the presence of TLR-9 protein in primary epithelial cell cultures of chronic rhinosinusitis with nasal polyps(CRSwNP),and then explore the role of innate immune recognition in the pathogenesis of CRSwNP.Method:Primary epithelial cell cultures were established in 10 controls and 10 CRSwNP patients who underwent sinus surgery,and flow cytometry was used to confirm its purity and measure the expression of TLR-9 protein.Result: By digestive method 98% of cultured primary nasal mucosa cells were epithelial cells and the expression of TLR-9 protein in CRSwNP group(11%-15%) was lower than that in normal group(49%-60%).Conclusion:This finding suggests that impaired innate immune responses via TLR-9 on sinonasal epithelial cells may involve in pathogenesis of CRSwNP.