乌司他丁对大鼠内毒素性急性肺损伤保护作用机制的研究

目的：研究乌司他丁（UTI）对大鼠内毒素性急性肺损伤（ALI）保护作用机制.方法：采用大鼠内毒素ALI模型,分别观察生理盐水对照组（NS组）、内毒素组（LPS组）、乌司他丁治疗组（UTI组）于LPS滴注4 h后动脉血气,血浆IL-8、MDA含量及测定肺湿/干重比.结果：LPS组、UTI组分别与NS组比较,其血浆IL-8、MDA、PaCO2以及肺湿/干重比都明显升高,PaO2明显降低且UTI组各观察指标值的变化小于LPS组,差异具有显著性.结论：乌司他丁对大鼠内毒素性急性肺损伤具有保护作用,机制可能与减少炎性介质与氧自由基的释放,抑制酶联反应有关.

The Protective Effects of Ulinastatin on Lipopolysaccharide-induced Acute Lung Injury in Rats and Its Mechanisms

Objective:To investigate the effects of urinastatin on lipopolysaccharide-induced acute lung injury in rats and its mechanisms.Methods:Thirty Wistar rats were divided randomly into saline control(NS group),lipopolysaccharide group(LPS group),urinastatin for treatment group(UTI group).Models of endotoxin induced ALI were used to observe the indexes of lung wet/dry weight.Arterial blood was drawn for blood gas analysis.Interleukin-8(IL-8) and Malonaldehyde Acetal(MDA) were assessed 4 hours after the lipopolysaccharide injection.Results:Compared with the NS group,the W/D,the content of MDA and IL-8 were significantly increased(P<0.01) and there was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in the LPS group(P<0.01);But in urinastatin for treatment group,these indexes of lung injury were significantly lower than those in the group of LPS(P<0.05).Conclusion:The high-expression of MDA and IL-8 should play important roles in lipopolysaccharide-induced acute lung injury.Urinastatin could protect lipopolysaccharide-induced acute lung injury by inhibiting the lung inflammatory injury.