KNT009安全性药理学研究

目的:观察KNT009对小鼠神经系统、犬心血管系统及呼吸系统的影响。方法:实验均分高、中、低剂量组及空白对照和阳性对照共5个组,小鼠实验给药组分别单次慢速静脉注射(iv)KNT009 160、80和40 mg/kg,加替沙星80 mg/kg,观察动物的自发活动和爬杆能力;犬实验给药组分别单次慢速iv KNT009 40、20 和10 mg/kg,加替沙星20 mg/kg,观察药物对犬血压、ECG、呼吸频率及节律的影响。结果:单次慢速iv KNT009对小鼠自发活动和爬杆能力无明显影响。单次慢速iv KNT009可降低犬收缩压、舒张压、平均动脉压,减慢心率,延长QT间期,使犬呼吸频率减慢,呼吸幅度加大,且均存在量效关系,3 h后上述指标基本恢复正常,加替沙星也有类似变化。结论:KNT009对心血管和呼吸系统有一定的影响,可使血压下降,心率减慢,QT间期延长,呼吸频率减慢,呼吸幅度加大。

Safety pharmacology study of KNT009

Objective:To study the effects of KNT009 on nervous, cardiovascular and respiratory system in animals. Methods: Mice were randomly divided to receive single 160, 80, 40, and 0 mg/kg dose of KNT009 and 80 mg/kg dose of gatigloxacin respectively and the spontaneous activities and pull-down ability of animals were observed. Dogs were randomly divided to receive single 40, 20, 10 and 0 mg/kg dose of KNT009 and 20 mg/kg dose of gatigloxacin respectively and the blood pressure, ECG , respiratory rate and width of dogs were measured. Results: No effects on spontaneous activities and pull-down ability in mice were observed. The systolic blood pressure, diastolic blood pressure, mean arterial pressure, heart rate and respiratory rate of dogs were decreased, the QT interval was prolonged and the respiratory width was increased in dogs, and there were dose effective relationship, however, these parameters return to normal within 3h. Conclusion: KNT009 has some effects on cardiovascular system in dogs such as the blood pressure and heart rate may be decreased and the QT interval may be prolonged, KNT009 also has some effects on respiratory system in dogs such as the respiratory rate may be decreased and the respiratory width may be increased.