放射免疫分析法相关参数对甲状腺球蛋白检测结果的影响

目的评价放射免疫分析法曲线拟合方式一定时,不同试剂盒、结合率方式(B/T与B/B0)对甲状腺球蛋白(TG)检测结果的影响。方法选用国内具有代表性的3家放射免疫试剂盒(A、B、C组),分别对78例临床确诊的甲状腺疾病患者血清TG进行检测。曲线拟合方式一定前提下,分别用B/T与B/B0 2种结合率方式进行检测,分析3家放射免疫试剂盒的不同结合率方式对检查结果的影响。结果 A、B、C组试剂盒检测TG值差异有统计学意义(z=6.11,P0.05);A组与B组检测TG值差异无统计学意义(z=0.894,P0.05);A组与C组检测TG值差异有统计学意义(z=2.01,P0.05);B组和C组检测TG值差异也有统计学意义(z=1.963,P0.05)。在B/T与B/B0 2种结合率方式下,A组和B组检测TG值差异均无统计学意义(P0.05),C组检测TG值差异有统计学意义(z=-1.68,P0.05)。结论曲线拟合方式一定,选择不同的放射免疫试剂盒必须选择其试剂盒所对应的结合率方式,才能得出较准确的TG检测结果。

Influence of radioimmunoassay related parameters on thyroglobulin detection results

Objective To evaluate the influence of different reagent kits and binding rate modes(B/T and B/B0) on thyroglobulin (TG) detection results in certain radioimmunoassay curve fitting way .Methods The domestic representative three kinds of radioimmunoassay reagent kits were selected(group A ,B and C) .Serum TG was detec‐ted in 78 cases of clinically diagnosed thyroid disease .Under the precondition of certain curve fitting way ,the detec‐tion was performed by using the two kinds of binding rate mode B/T and B/B0 for analyzing the influence of different binding rate mode in 3 kinds of radioimmunoassay reagent kits on the detection results .Results The detected TG values had statistically significant differences among the group A ,B and C(z=6 .11 ,P<0 .05);which had no statisti‐cally significant difference between the group A and B (z= 0 .894 ,P> 0 .05);which had statistically significant difference between the group A and C (z=2 .01 ,P< 0 .05);which also had statistically significant difference be‐tween the group B and C (z=1 .963 ,P<0 .05) .Under the binding rate modes of B/T and B/B0 ,the detected Tg val‐ue had no statistically significant difference between the group A and B (P>0 .05);the detected TG value in the group C had statistically significant difference (z= -1 .68 ,P<0 .05) .Conclusion In the certain curve fitting way , choosing different radioimmunoassay reagent kits must select their corresponding binding rate mode can obtain rela‐tively accurate TG detection results.