壳聚糖导管复合雪旺细胞修复兔面神经缺损的实验研究

目的：应用壳聚糖导管作为神经再生室, 复合体外培养的雪旺细胞修复兔面神经缺损,观察神经再生的效果。 方法： 用壳聚糖和羧甲基壳聚按3：1的比例采用冷冻干燥法制成壳聚糖复合导管； 取胎兔坐骨神经，经体外增殖培养获得雪旺细胞，复合于壳聚糖导管，修复兔面神经0.8?cm的缺损,并与单用壳聚糖导管作对照，观察修复后4、8、16周的神经电生理及组织学检查情况。结果:术后8周新生面神经纤维已沿管壁通过缺损到达远端，术后16周导管大部分降解，新生神经变粗变直，神经纤维排列整齐规则,神经电生理检查记录到复合动作电位，复合雪旺氏细胞的导管再生神经较粗，动作电位幅值较高。结论：壳聚糖导管可引导神经再生，复合雪旺细胞的壳聚糖导管神经再生效果较好。

An experimental study on repairing of facial nerve defects with

To evaluate the effect of complex chitosan conduits containing Schwann cells(SCs) in repairing of facial nerve defects. Methods: Complex chitosan conduits were made from chitosan and carboxymethyl chitosan by freeze drying method in proportion of 3 to 1. Schwann cells were obtained from sciatic nerves of new born rabbits, and then were cultured and number enlarged. After being combined with SCs, the complex chitosan conduits were used to repair a 0.8?cm defect of facial nerve. The nerve regeneration was evaluated by gross, electrophysiological andstological examinations after 4, 8 and 16 weeks, and were compared with the results of using conduits only. Results: New nerve had bridged the defects 8 weeks after operation, and most of the conduits had degraded 16 weeks after operation. Compared with chitosan conduits only , the conduits+ SCs group had better electrophysiological and histological results. Conclusion: The complex chitosan conduit combined with SCs is a better method for repairing of facial nerve defects.