大鼠背根神经节细胞的分离及特性探讨

目的探讨大鼠背根神经节（dorsal root ganglion,DRG）细胞的分离方法以及细胞形态和电生理特征。方法采用显微外科技术获取大鼠DRG体,用双酶法急性分离大鼠DRG获得DRG细胞,全细胞膜片钳技术记录动作电位和钠电流。结果本实验能得到完整圆形或椭圆长条形的大鼠DRG体。正常的单个DRG细胞呈圆形或椭圆形,大小不等,胞膜清晰,折光性好,隐约可见细胞核。在DRG细胞上记录的动作电位都具有从0期到4期,呈正立锐角三角形,静息电位小,动作电位时程短。DRG细胞的钠通道最大电流密度在-30mV左右,几乎能被,TTX完全抑制,具有可逆性恢复。结论本实验采用分离方法简单易行,DRG细胞容易获得和辨认,适合膜片钳技术要求,电生理特征明确可靠,值得推崇。

The isolation and characteristics of dorsal root ganglion neurons in rats

Objective To investigate isolation method and characteristics of morphology and electrophysiology in dorsal root ganglion neurons （DRG） of rats. Methods The bodies of dorsal root ganglion were isolated by microoperation. DRGs were rapidly obtained by double enzymatic dissociation. The whole-cell patch clamp was used to record action potentials （AID） and sodium currents （INa）. Results The results were illustrated that it was completely operated on bodies of round and long DRGs. APs recorded in DRG neurons were consisted of five phases from upstroke to phase four and shaped upward acute triangles, which were little rest potential and short action potential duration. INa recording DRG neurons were peak current density at clamp potential -30mV, and almost inhibited by TTX and recovered from inactivated state. Conclusions It was concluded that the operations on bodies of DRGs were simple and easy, and DRG neurons were rapidly gained and recognized in patch clamp experiments. The electrophysiological characteristics of DRG neurons were rather significant.