| 儿茶HPLC指纹图谱研究 |
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| 引用本文： | 李锐,陈国彪. 儿茶HPLC指纹图谱研究[J]. 广东药学院学报, 2011, 27(4): 388-391. DOI: 10.3969/j.issn.1006-8783.2011.04.015 |
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| 作者姓名： | 李锐 陈国彪 |
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| 作者单位： | 海南省药品检验所,海南海口,570216 |
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| 摘 要： | 目的 建立儿茶的HPLC指纹图谱.方法 采用Hypersil ODS2（150mm×4.6mm,5μm）柱,以乙腈-0.05％（体积分数）H3PO4溶液为流动相进行梯度洗脱,检测波长为265 nm,流速为1.0 mL/min,柱温为30℃.采用“中药色谱指纹图谱相似度评价系统（2004A版）”软件对其进行了相似度计算,...
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| 关 键 词： | 儿茶 指纹图谱 HPLC |
Studies on HPLC fingerprints of Acacia catechu |
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| LI Rui,CHEN Guo-biao. Studies on HPLC fingerprints of Acacia catechu[J]. Academic Journal of Guangdong College of Pharmacy, 2011, 27(4): 388-391. DOI: 10.3969/j.issn.1006-8783.2011.04.015 |
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| Authors: | LI Rui CHEN Guo-biao |
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| Affiliation: | (Hainan Provincial Institute for Drug Control,Haikou 570216,China) |
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| Abstract: | Objective To develop the fingerprints of Acacia catechu based on high performance liquid chromatography（HPLC）.Methods The HPLC separation was performed on a Hypersil ODS2（150 mm × 4.6 mm,5 μm）column by gradient eluting with methyl cyanide and 0.05% phosphoric acid at the flow rate of 1.0 mL/min.The UV detection wavelength was set at 265 nm,and the column temperature was maintained at 30 ℃.Similarity of fingerprint were calculated by sofware of ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004A＂,and Samples were clustered by SPSS sofware.Results Twelve common peaks were identified and the similarity of 10 batches of Acacia catechu was more than 95%.Conclusion The fingerprints established could be used for the quality control of Acacia catechu due to its high stability and good reproducibility. |
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| Keywords: | Acacia catechu fingerprints HPLC |
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