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The effect of ultra-nanocrystalline diamond films on the proliferation and differentiation of neural stem cells
Authors:Ying-Chieh Chen  Don-Ching Lee  Chao-Yang Hsiao  Yu-Fen Chung  Huang-Chin Chen  Joseph P. Thomas  Way-Faung Pong  Nyan-Hwa Tai  I.-Nan Lin  Ing-Ming Chiu
Affiliation:1. Department of Materials Science and Engineering, National Tsing-Hua University, Hsin-Chu 300, Taiwan;2. Institute of Cellular and System Medicine, National Health Research Institutes, 35 Keyan Road, Jhunan, Miaoli 35053, Taiwan;3. Department of Physics, Tamkang University, Tamsui 251, Taiwan;4. Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA;1. Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan;2. Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei, Taiwan;1. Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;2. Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084, PR China;3. Department of Chemistry and Center for Nanotechnology, Chung-Yuan Christian University, Chung-Li 32023, Taiwan;1. Department of Applied Science, National Hsinchu University of Education, Hsinchu 30014, Taiwan, ROC;2. Department of Materials Science and Engineering, National Tsing-Hua University, Hsinchu 30013, Taiwan, ROC;3. Department of Physics, Tamkang University, Tamsui 251, Taiwan, ROC;1. INSERM, UA01, Clinatec Laboratory, Biomedical Research Center Edmond J. Safra, F-38 000 Grenoble, France;2. Université Grenoble Alpes, UA01, Clinatec Laboratory, Biomedical Research Center Edmond J. Safra, F-38 000 Grenoble, France;3. CEA, LETI, Clinatec, F-38000 Grenoble, France;4. CEA, LIST, Diamond Sensors Laboratory, 91191 Gif-sur-Yvette, France;5. Thales Research & Technology, Palaiseau, 91767 Cedex, France;6. ESIEE-Paris, ESYCOM, University Paris EST, Cité Descartes BP99, Noisy-Le-Grand F-93160, France;7. Qwane Biosciences SA, EPFL Innovation Park, CH-1015 Lausanne, Switzerland;8. INSERM, U968, Institut de la Vision, Paris F-75012, France;9. Sorbonne Universités, UPMC Univ Paris 06, UMR_S968, Institut de la Vision, Paris F-75012, France;10. CNRS UMR7210, Institut de la Vision, Paris 75012, France;1. Department of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, USA;2. W.M. Keck Center for Collaborative Neuroscience, Department of Cell Biology and Neuroscience, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, USA;3. Department of Chemical and Biochemical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, USA;4. Center for Neuroscience, Shantou University Medical College, 22 Xin Ling Road, Shantou 515041, People''s Republic of China
Abstract:
The interaction of ultra-nanocrystalline diamond (UNCD) with neural stem cells (NSCs) has been studied along with its surface modification in order to improve its function as a biomaterial. Hydrogen- and oxygen-terminated UNCD films were compared with standard grade polystyrene in terms of their impact on the growth, expansion and differentiation of NSCs. When NSCs were cultured on these substrates in low serum and without any differentiating factors, hydrogen-terminated UNCD films spontaneously induced cell proliferation and neuronal differentiation. Oxygen-terminated UNCD films were also shown to further improve neural differentiation, with a preference to differentiate into oligodendrocytes. Hence, controlling the surface properties of UNCD could manipulate the differentiation of NSCs for different biomedical applications. These observations raise the potential for the use of UNCD as a biomaterial for central nervous system transplantation and tissue engineering.
Keywords:
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