The osteogenic differentiation of rat bone marrow stromal cells cultured with dexamethasone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles |
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| Authors: | Joaquim M. Oliveira Rui A. Sousa Noriko Kotobuki Mika Tadokoro Motohiro Hirose Jo?o F. Mano Rui L. Reis Hajime Ohgushi |
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| Affiliation: | 1. 3B''s Research Group – Biomaterials, Biodegradables and Biomimetics, Univ. Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, S. Cláudio de Barco, 4806-909 Taipas, Guimarães, Portugal;2. IBB – Institute for Biotechnology and Bioengineering, PT Government Associated Laboratory, Guimarães, Portugal;3. Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Nakoji 3-11-46, Amagasaki, 661-0974 Hyogo, Japan;1. College of Polymer Science and Engineering, Sichuan University, Yi Huan Road, South Section One, No. 24, Chengdu 610065, China;2. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610065, China;3. West China School of Stomatology, Sichuan University, Chengdu 610065, China;1. Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States;2. Department of Physical Sciences, Charleston Southern University, Charleston, SC 29423, United States;1. Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA;2. Joint Biomedical Engineering Program between The University of Texas at Arlington and The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;1. Key Laboratory of Advanced Technologies of Material, Minister of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China;2. School of Mechanical Engineering, Southwest Jiaotong University, Chengdu 610031, PR China;3. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China;1. College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China;2. State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China;1. Department of Oral and Maxillofacial Pathology, and Research Center for Tooth and Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, Seoul, Republic of Korea;2. Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea;3. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea;4. Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea;5. Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, Republic of Korea |
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| Abstract: | ![]()
There is an increasing interest in developing novel macromolecular vehicles for the intracellular and controlled delivery of bioactive molecules, since they can allow modulation of the cellular functions in a more effective manner ex vivo, and maintain the cellular phenotype in vivo upon re-implantation. The present study was designed to investigate the effect of combining novel dexamethasone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer (Dex-loaded CMCht/PAMAM) nanoparticles and, both HA and SPCL scaffolds (3D system) on the proliferation and osteogenic differentiation of rat bone marrow stromal cells (RBMSCs) in vitro. A luminescent cell viability assay using RBMSCs was performed for screening cytotoxicity of the developed HA and SPCL scaffolds. Results corroborated previous ones which have demonstrated in vitro, the superior performance of the HA and SPCL scaffolds on supporting cells adhesion and proliferation. Furthermore, this work showed that RBMSCs seeded onto the surface of both HA and SPCL scaffolds differentiate into osteoblasts when cultured in the presence of 0.01 mg ml?1 Dex-loaded CMCht/PAMAM dendrimer nanoparticles. In addition, results demonstrated that Dex-loaded CMCht/PAMAM dendrimer nanoparticles combined with the HA enhance osteogenesis by increasing ALP activity and mineralization of the extra-cellular matrix. The pre-incubation of stem cells with these kinds of nanoparticles allows the delivery of Dex inside the cells and directly influences their cellular fate, being a promising new tool to be used in cells and tissue engineering strategies. |
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