|
|||||
|
|
| 基因芯片技术在泌尿系统结核诊断中的应用 | |
| 引用本文： | 刘智勇,王丽馨,夏涵,张波,府伟灵. 基因芯片技术在泌尿系统结核诊断中的应用[J]. 中华医院感染学杂志, 2012, 22(16): 3465-3467 |
| 作者姓名： | 刘智勇 王丽馨 夏涵 张波 府伟灵 |
| 作者单位： | 1. 第三军医大学第一附属医院检验科,重庆,400038 2. 第三军医大学第三附属医院检验科,重庆,400042 |
| 基金项目： | 国家“863”项目(2011AA02A100);国家、军队重大专项(2012ZX10004801-003-006,AWS11C0012011);国家传染病重大专项分题2008ZX10003-012;国家自然科学基金项目(30927002 81071429,30901388) |
| 摘 要： | 目的 引入一种新方法对尿液中的结核分枝杆菌进行检测.方法 留取患者24 h尿液标本,离心,收集沉淀,并从中提取DNA,用晶芯分枝杆菌菌种鉴定试剂盒对样本DNA进行检测.结果 5例尿液中的结核分枝杆菌可以被基因芯片检测出,用以辅助抗痨治疗.结论 用基因芯片方法对尿液中的结核分枝杆菌进行快速检测是可行的. |
| 关 键 词： | 基因芯片技术 泌尿系 结核 诊断 |
Gene chip technique in diagnosis of urinary tract tuberculosis |
|
| LIU Zhi-yong , WANG Li-xin , XIA Han , ZHANG Bo , FU Wei-ling. Gene chip technique in diagnosis of urinary tract tuberculosis[J]. Chinese Journal of Nosocomiology, 2012, 22(16): 3465-3467 | |
| Authors: | LIU Zhi-yong WANG Li-xin XIA Han ZHANG Bo FU Wei-ling |
| Affiliation: | (Southwest Hospital,The Third Military Medical University,Chongqing 400038,China) |
| Abstract: | OBJECTIVE To introduce a new approach to detect Mycobacterium tuberculosis in the urine.METHODS The urine specimens within 24h were collected from the patients and then were centrifuged to get the pellets.Then the DNA of the pellets was extracted and detected by CapitalBio mycobacteria identification array kit.RESULTS M.tuberculosis in the 5 urine samples could be detected by the gene chip technique,the detection result could provide guidance for appropriate treatment.CONCLUSION It is feasible and reliable to rapidly detect M.tuberculosis in urine by the gene chip technique. |
| Keywords: | Gene chip technique Urinary system Tuberculosis Diagosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |