ICU肠球菌属esp基因分布及对万古霉素耐药的相关性研究

目的 了解医院重症监护病房(ICU)来源的肠球菌属的种类、esp基因的分布及其对万古霉素耐药的相关性.方法 用法国生物梅里埃公司全自动细菌分析仪VITEK-32进行肠球菌属鉴定,菌落杂交法检测esp基因的分布,微量稀释法测定细菌对万古霉素和替考拉宁的MIC.结果 113株肠球菌属中以粪肠球菌和屎肠球菌为主,共94株占总数的83.2％,其中粪肠球菌63株占55.8％,屎肠球菌31株占27.4％,esp基因阳性72株占76.6％,esp基因阳性者对万古霉素和替考拉宁的耐药率明显高于esp基因阴性者.结论 ICU感染肠球菌属以粪肠球菌和屎肠球菌为主,而且esp基因阳性较高,与肠球菌属耐万古霉素密切相关,值得重视.

Relationship between espgene in Enterococcus from ICU and vancomycin resistance

OBJECTIVE To study the species of Enterococcus from ICU and the relationship between esp gene and vancomycin resistance.METHODS Enterococcus isolates were identified by using fully automatic bacterial analyzer VITEK-32 of BioMérieux,France,esp genes were detected by colony hybridization method,and MIC of vancomycin and teicoplanin were determined by broth micro-dilution method.RESULTS Of 113 Enterococcus isolates,there were totally 94 strains of E.faecalis and E.faecium,accounting for 83.2%,including 63 strains of E.faecalis(55.8%) and 31 strains of E.faecium(27.4%),72 strains with positive esp genes accounted for 76.6%;the resistance rates of esp gene-positive strains to vancomycin and teicoplanin were significantly higher than those of esp gene-negative isolates.CONCLUSION The main Enterococcus strains isolated from ICU are E.faecalis and E.faecium,the high esp gene-positive is closely associated with vancomycin resistance in Enterococcus.