游泳训练对脑出血大鼠认知功能及NMDAR表达的影响

目的 探讨游泳对脑出血(ICH)大鼠认知功能的影响,并分析其相关的神经生物学机制.方法 将30只Sprague-Dawley大鼠采用随机数字表法分为3组:假手术组(假手术组),脑出血模型组(模型组),脑出血后游泳组(游泳组);每组10只.模型组和游泳组,将I型胶原酶注入大鼠尾状核建立ICH大鼠模型;造模成功后1 d,游...

Effects of swimming training on cognitive function and NMDAR expression in rats with cerebral hemorrhage

Objective To explore the effect of swimming training on cognitive function in rats with intracerebral hemorrhage (ICH) and to analyze its related neurobiological mechanism. Methods A total of 30 Sprague-Dawley rats were randomly divided into three groups:sham-operatedgroup, intracerebral hemorrhage model group and swimming group, undergoing swimming training after ICH. The ICH model was induced by infusion of type I collagenase into the caudatenucleus of rats. The neurological function was evaluated by a modified neurological severity score (mNSS) after 1d of successful model building and 1 d, 3 d, 5 d of swimming training. Morris water maze (MWM) test was performed at the same time in three groups to evaluate thecognitive function in rats after swimming training. After MWM detection, the rats were sacrificed and immunohistochemical staining was used to evaluate the expression of N-methyl-d-aspartate receptor (NMDAR) in hippocampus of rats. The expressions of N-methyl-d-aspartate receptor 1 (NR1) and N-methyl-d-aspartate receptor 2B(NR2B) in CA3 region of the hippocampus were evaluated by Western blot. Results Two-factor repeated measure ANOVA showed thatcompared with the model group, mNSS scores in the swimming group were lower (P<0.05), and the scores of the model group and the swimming group decreased over time (P>0.05), and there were no significant differences in inter-group and temporal interactions (P<0.05). Compared with the model group, the escape latency of the swimming group was longer (P<0.05), with less target quadrant time proportion (P<0.05), and the escape latency of the model group and the swimming group decreased with the change of time, the proportion of target quadrant time increased (P<0.05), and there were no significant differences in inter-group and temporal interactions (P>0.05).Immunohistochemical staining analysis showed that compared with the model group, swimming training significantly decreased the expression of NR1 and NR2B in CA3 region of the contralateral hippocampus (P<0.05).Western blot analysis showed that compared with the model group, swimming training significantly decreased the expressionof NR1 and NR2B in CA3 region of the contralateral hippocampus (P<0.05). Conclusion Swimming training can improve the cognitive function of ICH rats by down-regulating the expression of NR1 and NR2B in hippocampal CA3 region.