脂质体与完全弗氏佐剂预防NOD鼠胰岛炎探讨

目的 :探讨完全弗氏佐剂 (CFA)、不完全弗氏佐剂 (IFA)、大复层脂质体 (LML)、小单层阳离子脂质体(DOTAP)对NOD鼠胰岛炎和糖尿病发病的影响。方法 :①将 3周龄的NOD雌鼠随机分为 3组 ,分别给予CFA后脚板注射 (n =16 ) ,IFA和PBS乳化后腹腔注射 (n =16 ) ,单独PBS后脚板注射 (n =16 )。观察 12周龄胰岛病理及 30周龄糖尿病发病情况。②将 3周龄的NOD雌鼠随机分为 3组 ,分别给予大复层脂质体 (LML) (n =13) ,小单层阳离子脂质体 (DOTAP) (n =13) ,单独PBS腹腔注射 (n =13) ,同上观察胰岛病理及糖尿病发病情况。结果 :①CFA后脚板注射能明显降低胰岛炎计分 ,减少糖尿病的发生。 30周龄时 ,CFA组的发病率明显低于PBS后脚板注射对照组 (分别为9 2 %和 81 8% ,P =0 .0 0 1)。 12周龄时 ,CFA组胰岛炎计分明显低于对照组 [分别为 (0 0 5± 0 0 2 )和 (0 5 4± 0 11) ,P<0 .0 0 1]。②LML腹腔注射能明显降低胰岛炎计分。 12周龄时 ,LML组胰岛炎计分明显低于PBS腹腔注射对照组 [分别为 (0 16± 0 0 2 )和 (0 5 8± 0 0 6 ) ,P <0 .0 0 1]。 30周龄时 ,LML组糖尿病发生率为 6 0 % ,对照组为 90 % ,两组间无统计学差异。③在预防胰岛炎的作用上 ,CFA优于LML。结论 :CFA后脚板注射可预防NOD鼠发生糖尿病并减

Liposome and complete Freund''s adjuvant for the prevention of insulitis in non-obese diabetic mice

Objective To explore the effects of complete Freund’s adjuvant (CFA), incomplete Freund’s adjuvant(IFA), large multilamellar liposome (LML) and small cationic liposome (DOTAP) on insulitis and diabetes. Methods ①3 week old non obese diabetic(NOD) female mice were randomly divided into 3 groups: CFA group (injected subcutaneously in the hind footpad, n=16), IFA group (injected intraperitoneally, n=16) and PBS group (injected subcutaneously in the hind footpad, n=16). Three mice from each group (9 in total) were killed at the age of 12 weeks for the analysis of pancreatic pathology, and the others were not killed until they were 30 weeks old for diabetes incidence. ②3 week old NOD female mice were randomly divided into 3 groups and injected intraperitoneally with LML (n=13), DOTAP (n=13) and PBS (n=13), respectively. The insulitis score and diabetes incidence were estimated in the same way. Results ①CFA injected subcutaneously in the hind footpad could significantly reduce the insulitis score and decrease diabetes incidence in NOD mice. At the age of 30 weeks, the incidence of diabetes in the CFA group was lower than that in the PBS group (injected subcutaneously in the hind footpad) (9.2% vs 81.8%,P=0.001). At the age of 12 weeks, the insulitis score in the CFA group was lower than that in the control group[(0.05±0.02) vs (0.54±0.11),P<0.001]. ②LML injected intraperitoneally could significantly reduce the insulitis score in NOD mice. At the age of 12 weeks, the insulitis score in the LML group was lower than that in the PBS control group (injected intraperitoneally)[(0.16±0.02) vs (0.58±0.06), P<0.001]. At the age of 30 weeks, there were no significant differences in the diabetes incidence between the LML group and the PBS group (60% vs 90%). ③The protective effect of CFA was better than that of LML in NOD mice. Conclusion CFA injected subcutaneously in the hind footpad may prevent NOD mice from developing diabetes and reduce the insulitis severity. Although the protective effect of CFA is better than that of LML, LML can lessen the insulitis severity and may become a new preventive strategy in NOD mice.